RE: [Histonet] Embedding cell pellets

2009-04-02 Thread Bernice Frederick
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Pier Sent: Thursday, April 02, 2009 11:18 AM To: histonet@lists.utsouthwestern.edu; louise_hart...@urmc.rochester.edu Subject: Re: [Histonet] Embedding cell pellets Fix the cells, spin them down

Re: [Histonet] Embedding cell pellets

2009-04-02 Thread anh2006
Histogel works well for this purpose too. --Original Message-- From: Thomas Pier Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu To: louise_hart...@urmc.rochester.edu Sent: Apr 2, 2009 12:17 PM Subject: Re: [Histonet] Embedding cell pellets Fix the

Re: [Histonet] Embedding cell pellets

2009-04-02 Thread Thomas Pier
Fix the cells, spin them down, decant, and resuspend in 1% agarose. The agar plug can be processed and embedded like any other piece of tissue. Tom Pier >>> "Hartson, Louise" 04/02/09 11:08 AM >>> I am looking for a protocol for embedding cell pellets in paraffin. Thanks, Louise Louise Harts