I use AbCam 32419 (rabbit monoclonal, clone F1) on the Bond at a dilution of 1:300 after EDTA retrieval; very clean, crisp results
Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Tuesday, October 05, 2010 1:07 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Fibronectin AB for human tissues Dear Histonetters! I've been looking for a good Fibronecting antibody to stain some human tumors for connective tissues. I've tried 6 from Abcam, all either not working or giving high background. if anyone knows of a good antibody, i would really appreciate it. Thank you. Igor Deyneko Infinity Pharmaceuticals Molecular Pathology Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet