Hopefully you removed the antifreeze (alcohol) before freezing? :-) I
have run into this a few times. I work in a core facility where people
send me samples from all over. Recently someone sent me some samples
fixed in Histofix, which is a commercially available aqueous fixative.
But they neglecte
The freezing point of water is 0 degrees C. The freezing point of 100%
ethanol is -114 degrees C. The freezing point of 70% alcohol is about -48
degrees C. Since most cryostats are at -20 to -25 degrees C, your tissue
isn't freezing completely. You probably have "slush" ice inside the cells.
No
