RE: [Histonet] Good Perfusion..."Sacrifice" vs "anesthesia"

2009-07-01 Thread JR R
com To: chana.de.w...@gmail.com; jf...@gladstone.ucsf.edu CC: histonet@lists.utsouthwestern.edu; rosenfeld...@hotmail.com Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP". It is hard to judge whether the animal

RE: Re: [Histonet] Good Perfusion...

2009-07-01 Thread gayle callis
estern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TF Sent: Tuesday, June 30, 2009 11:46 PM To: Chana de Wolf; jfish Cc: histonet; JR R Subject: Re: Re: [Histonet] Good Perfusion... I think many, many labs perfuse animals under deep anesthesia. Notice the word "DEEP&

Re: Re: [Histonet] Good Perfusion...

2009-07-01 Thread Merced M Leiker
Hey everyone...just thought I'd add an "official" word from the authorities in this debate...as I'd been taught or somehow led to believe otherwise: :-) Forwarded Message Date: Wednesday, July 01, 2009 11:19 AM -0400 From: IACUC To: Merced M Leiker Subject: RE: gener

Re: Re: [Histonet] Good Perfusion...

2009-06-30 Thread TF
7-01 03:42:30 收件人: jfish 抄送: histonet; JR R 主题: Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic c

Re: Re: [Histonet] Good Perfusion...

2009-06-30 Thread TF
let me say like this during the overdose injection, look into the animals' eyes. take their soul away. then the pre-mortem perfusion is humanized. 2009-07-01 TF 发件人: Chana de Wolf 发送时间: 2009-07-01 09:30:01 收件人: Ingles Claire 抄送: histonet 主题: Re: [Histonet] Good Perf

Re: [Histonet] Good Perfusion...

2009-06-30 Thread Chana de Wolf
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Chana de Wolf > Sent: Tue 6/30/2009 2:37 PM > To: jf...@gladstone.ucsf.edu > Cc: histonet@lists.utsouthwestern.edu; JR R > Subject: Re: [Histonet] Good Perfusion... > > > > Histonetters, > > Perfusion under deep anesthesia

RE: [Histonet] Good Perfusion...

2009-06-30 Thread Ingles Claire
f Chana de Wolf Sent: Tue 6/30/2009 2:37 PM To: jf...@gladstone.ucsf.edu Cc: histonet@lists.utsouthwestern.edu; JR R Subject: Re: [Histonet] Good Perfusion... Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is n

Re: [Histonet] Good Perfusion...

2009-06-30 Thread Chana de Wolf
Histonetters, Perfusion under deep anesthesia is most certainly not unethical NOR illegal, and, in fact (as mentioned by Jo Dee), it is necessary for optimal perfusion and fixation -- the intracellular ischemic cascade begins immediately upon circulatory arrest, setting off a chain of events highl

RE: [Histonet] Good Perfusion...

2009-06-30 Thread Jo Dee Fish
Dear Jerry and histonetters, I don't believe this is unethical or illegal. It is written into our IACUC approved protocols as follows: Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin or Halothane induced. Bilateral thoracotomy." Could it only be forbidden by your f