Obviously, you're going to get a much different result using a
polychromatic stain, than by doing a standard H&E. But if the pathologists
like it...
I'm not even sure if they could call that stain an H&E in their dictation.
Seems like that would open them up to liability if, God forbid, they ever
I would look at the dewaxing stage.
Have you changed waxes?
Try longer in xylene &/or longer in the oven.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, Un
Hi Charles,
If my memory serves, Clarifier 1 and Clarifier 2 are different mostly in that
one is an alcoholic solution and the other an aqueous one. So they both behave
differently in their mechanism of action. The alcohol based reagent should
remove more hematoxylin from the cells than the aqu
Usually inconsistency in H&E staining has two different sources: either the
staining process itself, or the previous tissue processing sequence.Your
staining protocol has a "standard" sequence but I would add an additional
xylene and would increase the time in hemtoxylin to 7 minutes for you wil
I am interested in the answer as I use this same technique on my rodent
samples and have not had this issue.
On 7/2/2013 8:07 AM, Connolly, Brett M wro
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in
formalin for 48 hrs. Since the tissues coul
We use Platinum Line hematoxylin and eosin from Mercedes Medical [MER4799 and
MER4131] - much cheaper than Richard-Allan and in a side-by-side blind test,
the pathologists actually preferred Platinum Line.
Tresa
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailt
I always used Richard Allan (Hematox; clarifier, bueing) and was always
satisfied.
René J.
--- On Tue, 4/3/12, Sheila Fonner wrote:
From: Sheila Fonner
Subject: [Histonet] H & E Staining
To: "Histonet"
Date: Tuesday, April 3, 2012, 9:51 AM
Good morning Histonetters,
Can anyone tell me if
a DeBrosse-Serra
To: 'Sheila Fonner' ; Histonet
Sent: Tuesday, April 3, 2012 10:05 AM
Subject: RE: [Histonet] H & E Staining
Sheila,
We are using the reagents from Leica, formerly Surgipath, Gill's II Hematoxylin
(01520) and Eosin (01520) on our Leica Autostainer. But there
Sheila,
We are using the reagents from Leica, formerly Surgipath, Gill's II Hematoxylin
(01520) and Eosin (01520) on our Leica Autostainer. But there are many other
vendors that are very good too. Way back when, we used to make our own
hematoxylin and eosin. But it really is so much easier to
