RE: [Histonet] How long ...

2010-09-20 Thread sgoebel
First off you shouldn't leave Bouin's sitting for that long out o= n a shelf. Once the water evaporates out of the solution it is extrem ely flammable (explosive even I think?). Also, you shouldn't leave ti= ssue in alcohol forever either because you will end up with "beef

Re: [Histonet] how long can slides stay in water or buffer

2009-02-23 Thread Mariya Peneva Dobreva
Never let them air dry! The staining won't work. They can stay in water quite some time, like hours. I even think they can stay 2-3 days at 4 degrees, but I'm not sure... With the buffers be careful! They can't stay in the buffers containing permeabilyzing agent (like tween20 or triton) longe