This is my understanding about this CAP checklist item -
CAP is looking for 2 types of negative controls:
1. Negative reagent control - this is satisfied by running a negative control
slide from the same patient block through the whole process without the primary
antibody.
2. Negative tissu
, Colorado 80504
-Original Message-
From: Amos Brooks [mailto:amosbro...@gmail.com]
Sent: Friday, May 20, 2011 3:05 PM
To: Liz Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
Thanks Liz,
You are absolutly right there, but have
Thanks Liz,
You are absolutly right there, but have you ever noticed some folks
eyes glaze over when you say that they must both be run at the same ug/mL? I
would be lying if I said it never happened to me until I forced myself to
work it out. Once you do though it isn't too bad. Unfortunately
Amos
Isotype negative controls are based upon protein concentration not
dilution. They must be the same protein concentration of the primary
antibody at the dilution you are using. Using the same dilution will
not work unless both stock solutions (primary antibody and isotype
control) are at the
l.com]
Sent: Friday, May 20, 2011 11:57 AM
To: Curt Tague
Cc: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
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Histonet@lists.utsouthwestern.edu
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AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
And the block in question has already been proven positive using THAT
procedure and antibody during validation.
Claire
From: histonet-boun...@lists.utsouthwestern.edu
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this no
sday, May 19, 2011 12:31 PM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a
essage-
From: pete.peder...@healthonecares.com [mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:31 PM
To: Dawson, Glen; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying con
ailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Martin
Lapointe
Sent: Friday, 20 May 2011 7:50 AM
To: Angela Bitting; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
I can certainly agree with that. Whether it’s the patient’s sample or the known
po
ailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pete.peder...@healthonecares.com
Sent: Friday, 20 May 2011 5:31 AM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correc
.@healthonecares.com
[mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:54 PM
To: Thomas Jasper; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Thomas,
Agreed, however, how can you say with certainty that the control is
still good,
...@copc.net]
Sent: Thursday, May 19, 2011 1:39 PM
To: Pedersen Pete; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are run
: Thursday, May 19, 2011 4:50 PM
To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
I agree with the majority. A patient slide stained without primary antibody,
and a patient section with primary antibody and a known positive con
...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn&#x
awson, Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
I agree 100% with Glen.
Jan Shivers
UMN VDL
- Original Message -
From: "Dawson, Glen"
To:
Sent: Thursday, May 19, 2011 1:32 PM
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patien
y 19, 2011 1:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense. Because it would not be from
the patient tissue being tested, ho
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
patient tissue being tested, how do you know if it was handled the same as the
patient tissue? For example:
1) Were they processed the same way?
2
The ideal situation is as follows: a known (+) control with the patient's
tissue to make sure that the reaction worked, and a (-) using a section from
the patient's tissue to rule out any false (+).
René J.
From: Curt Tague
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 12:0
Curt,
Nonsense. The negative control is used to evaluate endogenous staining
in the *patient* tissue. Your Pathologist needs to do another residency at
clown college.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
__
That's a new one on me!
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
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