Melanie,
Who's your vendor for buying Novec 7000?
Maria Mejia
San Francisco, CA
Affymetrix
On Apr 17, 2013, at 10:08 AM, mwh...@mcleodhealth.org wrote:
> I just wanted to clarify that we don't use Novec 7000 for freezing of
> muscle tissue. We use it for other routine frozen sections on
> hum
I just wanted to clarify that we don't use Novec 7000 for freezing of
muscle tissue. We use it for other routine frozen sections on
humans.Sorry if that caused any confusion. Thanks Tim for pointing that
out!
Melanie S. White, MT(ASCP)
Laboratory Supervisor, Systems/Anatomic Pathology
McLeo
, 2013 2:17 PM
To: 'Paula Sicurello'; HistoNet
Subject: RE: [Histonet] Isopentane storage-the polling is open
We store at room temperature in a flammable cabinet.
Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a
container of isopentane in a container of Liqui
For almost two years, we have been using- with great success- a much safer
alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a
paid endorser and these are my views only , and do not reflect in any way
on my employer. Our pathologists greatly prefer the results of a freezing
ba
day, April 16, 2013 2:32 PM
To: Paula Sicurello ; HistoNet
Subject: RE: [Histonet] Isopentane storage-the polling is open
Paula, we keep ours at room temp in a small flam cab near the muscle bench.
We keep two gallons on hand.
In Dapson & Dapson's book "Hazardous Materials in the Hi
We stored at RT but lab did not have a flammable storage cabinet .Since
we did not have an explosion proof refrigerator or cold room, it was deemed
too dangerous to store in those areas.
There is good reason why one doesn't store in regular refrigerators/cold
room. Histologic, Vol. XX
Hello Paula,
At my previous lab at UCSF, we often had a lot of large & small tissue to
freeze &
therefore we used quite a bit of liquid nitrogen. We would get our LN2 from a
local
vendo, & was delivered in a low pressure 230 tall slender LN2 tank. The tank
had a nozzle attached for easy & saf
We store at room temperature in a flammable cabinet.
Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing
a container of isopentane in a container of Liquid Nitrogen.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244
Paula, we keep ours at room temp in a small flam cab near the muscle bench. We
keep two gallons on hand.
In Dapson & Dapson's book "Hazardous Materials in the Histopathology
Laboratory" (Anatech, 2005) they do not make any specific recommendations
concerning storage temperature, except to say
Hi Julia,
Thanks for the information.
Can you tell me why you store it in the refrigerator?
Paula
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: 919.684.2091
On Tue,
We maintain an approximately 1 liter supply of isopentane and store all
isopentane (open/unopened) in an explosion proof freezer.
Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory
600 Highland Avenue
Madison, WI 53792-5132
-Original Message--
We store our open bottle in an explosion proof fridge and unopened at room temp
in a flammable storage cabinet
Julia Celebre Sr MLT
Anatomic Pathology
Hamilton General Hospital
905-527-4322 ext 46179
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet
> I have found no better substitue for isopentane
ditto with isopentane (methylbutane) here... -160 in liquid form...
though it will begin to freeze solid after several minutes...
___
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145, AUSTRALIA
-Original Message-
From: Joanne Mauger [mailto:mau...@email.chop.edu]
Sent: Thursday, 18 December 2008 12:50 AM
To: Tony Henwood; carmen_loise...@hotmail.com;
histo...@pathology.swmed.edu; sette...@umdnj.edu
Subject: RE: [Histonet] Isopentane
In my experience, liquid ni
ffect, creating an insulating gas layer between the
> tissue
> and the liquid and delaying the speed of freezing = an increase in ice
> crystal formation.
> Regards,
> Markus F. Meyenhofer
>
> - Original Message -
> From: "Tony Henwood"
> To: "Dana Sett
: antho...@chw.edu.au; carmen_loise...@hotmail.com;
histo...@pathology.swmed.edu; sette...@umdnj.edu
Subject: RE: [Histonet] Isopentane
In my experience, liquid nitrogen makes a lot of freezing artifact in
muscle tissue. I have found no better substitue for isopentane if you
want quality morphology
SW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Wednesday, 17 December 2008 5:27 AM
To: carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane
Hell
From: Markus F. Meyenhofer [mailto:mi...@superlink.net]
Sent: Wednesday, 17 December 2008 1:47 PM
To: Tony Henwood; Dana Settembre; carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane
Isopentane is cooled by liquid N2!
To freeze tissue direct in LN2 is not the best way to f
F. Meyenhofer
- Original Message -
From: "Tony Henwood"
To: "Dana Settembre" ; "carmen loiselle"
; "pathology education"
Sent: Tuesday, December 16, 2008 6:19 PM
Subject: RE: [Histonet] Isopentane
Try liquid nitrogen
Regards
Tony Henwood J
AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana
Settembre
Sent: Wednesday, 17 December 2008 5:27 AM
To: carmen loiselle; pathology education
Subject: Re: [Histonet] Isopentane
Hello Carmen
Hello Carmen,
Sorry I have no alternative. I know most labs use that.
When I worked in a neuropathology lab the isopentane was kept in the
refrigerator - an explosion proof one.
I believe that means there's no lightbulb inside and no inner working
of a lightbulb inside either.
Dana Settembre, HT
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