RE: [Histonet] Losing tissue on IHC slides

2013-07-29 Thread Sue Hunter
Good Morning Another thing to try is different slides. We have had issues with bad batches/lots of slides - even the Fisher Superfrost plus. Especially if your purchasing department buys in quantity and then stores the slides in hot warehouses. This seems to negate the charge on the slides -

RE: [Histonet] Losing tissue on IHC slides

2013-07-28 Thread Clare Thornton
I have a couple of things. First off, are the sections coming off the slides entirely, or does it have a "chewed up" appearance? If the sections are coming completely off, I would agree with the others that water is the issue. Also, make sure you are cutting no thicker than 4 microns. We had

Re: [Histonet] Losing tissue on IHC slides

2013-07-27 Thread Benjamin
So the patient tissue and control are on the same slide, and the control is staying on but patient tissue is falling off? Maybe dont bake the control tissue just let them air dry, the humidity in the slide drying oven might be messing with the super frost plus slides. Its just a coating on the s

Re: [Histonet] Losing tissue on IHC slides

2013-07-26 Thread Hans B Snyder
I agree water is always a potential issue. Tissue can be finicky though. Have you tried poly-L-lysine coated slides? They do help is some occasions. If you need a protocol, let me know, I can write one up, very easy. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7

Re: [Histonet] Losing tissue on IHC slides

2013-07-26 Thread Tony Auge
How are you drying your slides? If in an oven, what's the temp and duration? Try going longer and tap off excess water before drying. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.uts