>>At 19:19 03/03/2015, you wrote:
>>Hi!
>>For lacking Eosin-staining check the pH of the solution. It should
be 4-5.
>>Gudrun
>>= = =
Thanks for the tip! Nice to know it... after centuries doing stainings
:-)
Pablo
On 03/03/15, Emily Brown wrote:
> Hello!
>
Hi Emily,
My $0. 02 by saying it's a challenge for research groups, like ours, to keep
quality stains that are not used everyday/week/month.
I agree with your intuition. Buy or make new eosin.
As for stocking eosin (or hematoxylin), I buy premade by a reputable vendor and
always keep an ey
If you can't get two colours with H&E, don't expect to get the colour scheme
right with Masson's trichrome, which needs more skill. If you are hoping to
show basement membranes in the kidney, you would do better to use a technically
simpler staining method such as picro-sirius red or periodic ac
] On Behalf Of Laurie Colbert
Sent: Tuesday, March 03, 2015 9:46 AM
To: Emily Brown; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Masson trichrome and H and E
Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around
$60.00.
Laurie Colbert, HT (ASCP
Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around
$60.00.
Laurie Colbert, HT (ASCP)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015
We have been very satisfied with special stain solutions from Poly Scientific
(http://www.polyrnd.com/products/stains/general/aniline-blue---masson's-trichrome.aspx).
We get an 8oz bottle for under $30. Very good customer service too.
Toni
-Original Message-
From: histonet-boun...@lists
