Candice,
Remember freshly prepared Oil Red O Stock usually does not work.
Prepare your saturated Oil Red O Stock and leave it for a week or more before
using it to prepare a working solution.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senio
That would be great!
Thanks
I remain yours truely,
Candice Camille
From: Elizabeth Chlipala
To: 'Candice Smoots' ; Histonet
Sent: Wednesday, January 25, 2012 10:43 AM
Subject: RE: [Histonet] Oil red O versus Sudan 4
Candice
Most of the
: Candice Smoots
Sent: Wednesday, January 25, 2012 10:32 AM
Subject: RE: [Histonet] Oil red O versus Sudan 4
I do oil red a lot. You are doing it on frozen sections right?
What are you coverslipping with?
Need a few more details of the protocol and I might be able to help =)
Sarah Goebel-Dysart
Candice
Most of the references regarding this technique use Sudan IV, that's what we
use here. We use Oil Red O for the Aortic Root Sections but not for the En Face
Analysis of the Aorta. I have the Sudan IV procedure we use here if you would
like it.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)
