> Happy New Year, * John Kiernan*
> = = =
> --
> *From:* Haas, Elizabeth via Histonet
> *Sent:* 31 December 2019 11:46
> *To:* S hay
> *Cc:* histonet@lists.utsouthwestern.edu >
> *Subject:* Re: [Histonet] Pap stains
>
>
> I believe filtering stains daily is a CAP requirement
&
practice.
Happy New Year, John Kiernan
= = =
From: Haas, Elizabeth via Histonet
Sent: 31 December 2019 11:46
To: S hay
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Pap stains
I believe filtering stains daily is a CAP requirement
Sent from my iPhone
I believe filtering stains daily is a CAP requirement
Sent from my iPhone
> On Dec 31, 2019, at 9:23 AM, S hay via Histonet
> wrote:
>
> 1. Does everyone filter their pap stains daily?
> 2. Are you chaining all other reagents daily?
>
> Thanks in advance.
> __
Charles, What do you mean by "dark nuclei"? Are you asking about the normal
colour for the method, or about something you have not seen before that looks
wrong? Also, what is "PAP stain"?
If "PAP stain" means Papanicoloau, the nuclear stain is Mayer's haemalum. This
is a progressive stain; y
There are many reasons why the nuclei may look dark in the Pap stain.
1. What type of hematoxylin are you using?
2. What type of Pap stain are you using, regressive or progressive?
This could be due to a variety of things like: type of preparation utilized,
too long in hematoxylin, not enough rin
Are you referring to a Pap stain control? If so, we run a self made buccal
smear every day to check for stain quality.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comf
Xylene is becoming more and more of a nuisance material and a problem
for us in use and in disposal. We are still able to use it but with
increasing concern.
We have been able to eliminate xylene from our staining procedures
altogether in our small laboratory.
We use a hair dryer to dry the
Mike,
As an alternative for your discussion:
I work on medical mission trips where I run Paps in a field setting. For the
past 4 years, we have not had access to xylene, and we have done the following:
1. Remove slides from the last alcohol after staining
2. Allow to air dry completely (gener
Hi Mike:The steps you desrcibe are wrong.After you finish staining your PAP
smear, just wash them in the last ethanol → oven dry at 60ºC for 5 minutes or
as required if the smear is too thick and when completely dried →
coverslip.This final drying has to take place at temperatures above room tem
Thanks for these excellent comments on troubleshooting the EA and OG-6
components I've copied them for my files. The important thing is to have
frequent feedback from the cytotechnologist to whoever is doing the
staining.
In one job I had the staining was done by a clerical person who took great
p
Hi Charles - Not to worry. Many of us Histo folks don't have a Cytologist to
help. Beth is spot on in her advice. I just wanted to add Orange G should
appear yellow to orange; 15 sec to 1 minute is the usual range of staining
times. EA is often problematic because of fundamental limitations
Charles Riley HT(ASCP)CM, Histopathology Coordinator/ Mohs, Doctors
Pathology Services, Dover DE asks:
>>Not sure if anyone out there would know the answer to this. We are having
an issue with our PAP stained slides appearing too orange and looking aged.
If you have any idea for causes I appreciat
You need to review the slides fixation and what chemicals you are using
Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent
from my cell phone Original message From: Charles Riley via
Histonet Date: 2/4/2016 5:50 PM
(GMT+03:00) To: histonet@li
We just leave the pap pen on. Since it's not covering the tissue, it won't
matter.
Emily
"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."
-Chuck Palahniuk, "Haunt
This is our method:
1. Tap water dip gently 5-10 times
2. Harris haematoxylin 5 minutes
3. Wash in running water
4. Differentiate in acid/alcohol one dip
5. Wash in running water
6. Scott's Blueing So
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