Forget the floor damage.
You don't use xylene because it's a suspected carcinogen and it isomerizes
to free benzene, a known carcinogen, when it evaporates.
Xylene vapors are heavier than air and stay in your reserve lung volume and
do The Bad Thing.
NEVER use xylene for cleaning surfaces. Not
Paraffin buildup on floors. Histology's worst. Problem solution:
1. Everyday, sweep and scrape personal work areas - microtomes and embedding
2. Put paraffin catching mats at key traffic areas - Change every 2 weeks
3. Have Environmental Services/Housekeeping strip and re-wax the worst traffic
p
These work pretty well to keep wax from being spread around too much.
https://www.grainger.com/product/PLASTICOVER-Floor-Protection-Mats-45MT85
Place them strategically in thresholds and high traffic areas.
Unlike the blue semi-permanent ones you mentioned, which break down, when
these get dirt
After a ba-zillion years in Histology, I've found there is no substitution for
having the floors stripped every 2 weeks. The trick is NOT to apply floor wax
after stripping. We've also been currently using mats in key areas where the
most traffic is. This prevents wax from being ground into t
The Most venerable Histologist ( JK) is correct...as always!
No way to go from 70% alcohol directly into Pwax
Have to remove ALL water before infiltrating with Pwax
Yesalso need to replace alcohol with Xylene/Histoclear before infil. with
Pwax.
Sure, you can use Isopropyl alc to obviate xylene
Of course you are right!
This is yet another example of an error in a procedure informally handed on
from person to person! Always work from a book. Even a very old one will be OK
for paraffin embedding.
John Kiernan.
https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html
=
The processor screen temperature reflects the actual reading of an embedded
electronic temperature probe and, as such, will be more accurate (always at the
same place, in the same way, with constant precision) something rarely obtained
manually. Therefore, use the temperature reading from the sc
Hi Merissa,
As far as I am aware, insufficient paraffin infiltration would only affect
sectioning. The epitopes that we we are attempting to stain with IHC are
affected by pre-analytic factors such as fixation, cold ischemic time and
perhaps heat (too much) but plus or minus wax should not be an is
Have you tried using RDO on the block surface?
On Aug 12, 2015 12:30 PM, Jennifer Connell via Histonet
wrote:
Hello,
I am having trouble with sectioning of paraffin embedded human aortic valve
samples.
I received the embedded samples from a collaborator who had the embedding done
by the patholo
: "Arbaugh, Roberta"
Cc: "histonet@lists.utsouthwestern.edu"
Subject: Re: [Histonet] Paraffin block disposal
Message-ID: <1f36aba5-452b-4556-8d1e-e5d09fdb2...@gmail.com>
Content-Type: text/plain; charset=us-ascii
That's a good question. I'd like to know the answer
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu
-Original Message-
From: Mayer,Toysha N [mailto:tnma...@mdanderson.org]
Sent: Monday, June 08, 2015 9:02 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] : Paraffin block disposal
The regulation says two
edu"
Subject: Re: [Histonet] Paraffin block disposal
Message-ID: <1f36aba5-452b-4556-8d1e-e5d09fdb2...@gmail.com>
Content-Type: text/plain; charset=us-ascii
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun
Pensacola, FL 32514
Phone 850.474.8581
Fax 850.474.8584
-Original Message-
From: "Cooper, Brian"
To: a.tolentin...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Date: 06/06/2015 13:34
Subject: Re: [Histonet] Paraffin block disposal
Hey Aimee,
This has been discuss
10:25AM
To: Arbaugh, Roberta [rarba...@csdermatology.com]
CC: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] Paraffin block disposal
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun 5, 2015, at 12:54 PM,
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta
> wrote:
>
> Per CLIA we only need to keep paraffin blocks two years. What is the proper
> way to dispose of them?
>
> DISCLAIMER: The information in thi
the flotation bath.
Good Luck!
Sorin MusatThemis PathologyBucharest, RomaniaformerlyHistoBest Inc.Edmonton,
Alberta
From: Emily Brown
To: "Samala, Ramakrishna"
Cc: "histonet@lists.utsouthwestern.edu"
Sent: Friday, April 10, 2015 7:17 PM
Subject: Re: [Histonet] P
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
Sent: Thursday, February 05, 2015 10:22 AM
To: James Watson
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] paraffin sectioning-dry tissue?
By adding water, ice, or warm humidity (through exhalations) to the mix,
though
al Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller
> Sent: Thursday, February 05, 2015 6:41 AM
> To: Geoff
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] paraffin sec
: Re: [Histonet] paraffin sectioning-dry tissue?
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can
be really dry and needs a 'soak'. I have left them for an hour before now but
don't leave it for longer than 4 hours though because it can start to swell
Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can
be really dry and needs a 'soak'. I have left them for an hour before now but
don't leave it for longer than 4 hours though because it can start to swell and
de-process!
You will still only get a few non-chattery sec
This is common with mouse and rat tissues, they get "over-dried" with a
typical processing schedule.
Soaking the face of the block with a kimwipe wet with ice water for 60
-120 seconds will enable you to cut 10 nice sections, maybe more.
Geoff
On 2/5/2015 6:23 AM, Emily Brown wrote:
Hello all
After macro trimming into our blocks (very gently if friable or dry) we
place them on an ice tray to soak for a good while.
Each well is filled with a little bit of water - this rehydrates the
tissue just enough to get a few good sections off the top.
we¹ve had success with this method for most any
I also noticed stain fading with time - controls cut way ahead of staining?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com
On 1/23/15, 1:00 PM, "Wheelock, Timothy R."
wrote:
>Hi Jeffery:
>
>Yes, I notice th
beth
Chlipala
Sent: Friday, October 24, 2014 3:53 AM
To: Cooper, Brian; WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Paraffin Used
We do the same here.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060
: RE: [Histonet] Paraffin Used
Hi Bill,
We infiltrate with Paraplast, and embed with Paraplast Extra.
Thanks,
Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 900
Hi Bill,
We infiltrate with Paraplast, and embed with Paraplast Extra.
Thanks,
Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-20
, 2014 4:56 PM
To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet
Subject: RE: [Histonet] Paraffin blocks
I have been following this stream of conversation and thought I might jump in.
Paraffin blocks and microtomy waste are NOT biohazard waste. The process of
fixation
ESALVO [mailto:wdesalvo@outlook.com]
Sent: Thursday, July 10, 2014 1:56 PM
To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet
Subject: RE: [Histonet] Paraffin blocks
I have been following this stream of conversation and thought I might jump in.
Paraffin blocks and microtomy waste are NOT
; histonet
Subject: RE: [Histonet] Paraffin blocks
I have been following this stream of conversation and thought I might jump in.
Paraffin blocks and microtomy waste are NOT biohazard waste. The process of
fixation and tissue processing makes the tissue acceptable for general waste
disposal. You
for your blocks and cutting waste.
William DeSalvo, BS HTL(ASCP)
> From: vriv...@westderm.com
> To: bcoo...@chla.usc.edu; jfra...@hotmail.com;
> histonet@lists.utsouthwestern.edu
> Date: Thu, 10 Jul 2014 20:37:39 +
> Subject: RE: [Histonet] Paraffin blocks
> CC:
&g
visor
West Dermatology Pathology Laboratory
vriv...@westderm.com
714-924-7240 (Lab)
714-390-0906 (Cell)
-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu]
Sent: Thursday, July 10, 2014 1:30 PM
To: Vincent Rivera; JOSEPH FRAZEE; histonet server
Subject: RE: [Histonet] Paraf
Guess we're not so
linear . . .
Thanks,
Brian
-Original Message-
From: Vincent Rivera [mailto:vriv...@westderm.com]
Sent: Thursday, July 10, 2014 1:12 PM
To: Cooper, Brian; JOSEPH FRAZEE; histonet server
Subject: RE: [Histonet] Paraffin blocks
I know this is slightly off topic
:23 PM
To: JOSEPH FRAZEE; histonet server
Subject: RE: [Histonet] Paraffin blocks
In every institution I've ever worked, paraffin tissue blocks were handled as
regulated medical waste, and therefore discarded into red biohazard bags.
Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Departme
In every institution I've ever worked, paraffin tissue blocks were handled as
regulated medical waste, and therefore discarded into red biohazard bags.
Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
bcoo...@chl
We are currently using biohazard containers.
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-3982
mro...@sfmc.net
http://www.sfmc.net
-Original Message-
From: histonet-boun...@lists.utsouthweste
.pr...@tissueregenix.com
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 14 Jun 2013 07:45:47 +
> Subject: Re: [Histonet] Paraffin processing native sheep ACL
> CC:
>
> Hi Liz,
>
>
>
> I inherited the following protocol for ACL samples. It works quite well, but
Hi Liz,
I inherited the following protocol for ACL samples. It works quite well, but
times probably could be reduced - the optimisation is on my to-do list.
70% Alcohol - 1 hour
90% alcohol - 1 hour
100% Alcohol -2 hours
100% alcohol - 3 hours
100% alcohol - 4 hours
Xylene -
Michael,
Do you know or can you get the program they used to process the brain tissue?
Also are they whole or slices of brain (at what thciknees)?
Pam Marcum
- Original Message -
From: "Michael J. Lyon, Ph.D."
To: "Histonet"
Sent: Thursday, January 17, 2013 10:43:02 AM
It has been my experience that tissues that remain in paraffin too long (like
over a weekend) become brittle and hard. If we are embedding over 300 blocks,
those blocks may remain in the embedding station for up to 6 hours - but I
personally strongly recommend sticking to your SOP for processi
I have left them for over 24 hours with no ill effect on sectioning.
>>> Demetria Ross 9/27/2012 6:21 PM >>>
I'm curious to know how long can tissue stay on the machine in paraffin
before it becomes a problem I have left tissue stay in paraffin 30 min-2
hours before I take it off but not on a da
It also depends by the thickness of the pieces.
If they are small after about 20 minutes you can put them in new
paraffin where they will still stay for 20 minutes.
If the pieces have a medium thickness ( about a half of a cubic centimetre) it
would be better to keep them in the oven altogether for
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Friday, May 11, 2012 2:33 PM
To: 'gu.l...@gmx.at'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] paraffin melting in VIP
When it is time to exchange reagents, the paraffin is the fi
When it is time to exchange reagents, the paraffin is the first thing we do
because of the melting time required.
We fill up the bin and place it in the oven until there is room to add more wax
pellets - we do not stuff it in, but leave it rather loose. We do this about
six times during the day
-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, May 11, 2012 1:28 PM
To: histonet@lists.utsouthwestern.edu; gu.l...@gmx.at
Subject: Re: [Histonet] paraffin melting in VIP
I agree Rene!
I also believe Sakura recommends not putting paraffin flakes directly in
the containers.
On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa wrote:
> Regardless of the time it takes or of how many people do it, melting the
> paraffin directly in the VIP should not be done because it ca
Regardless of the time it takes or of how many people do it, melting the
paraffin directly in the VIP should not be done because it causes the heating
elements to work extra reducing their useful life. They are quite expensive to
replace!.
Melt the paraffin outside the VIP and use the VIP only
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 24, 2012 11:43 AM
To: histonet@lists.utsouthwestern.edu; E V
Subject: Re: [Histonet] Paraffin Block Shipping Question
I used to send the overnight in one of those Styrofoam containers along with
those freeze and reuse plastic pouches
I used to send the overnight in one of those Styrofoam containers along with
those freeze and reuse plastic pouches inside.
René J.
--- On Thu, 2/23/12, E V wrote:
From: E V
Subject: [Histonet] Paraffin Block Shipping Question
To: histonet@lists.utsouthwestern.edu
Date: Thursday, February 23,
.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor
Sent: Thursday, February 23, 2012 4:23 PM
To: hairlesstur...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin
O'Connor
[b427...@aol.com]
Sent: Thursday, February 23, 2012 4:23 PM
To: hairlesstur...@gmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Block Shipping Question
FedEx overnight with a warning to keep from extreme temps. That should keep
them off loading
FedEx overnight with a warning to keep from extreme temps. That should keep
them off loading docks.
-Original Message-
From: E V
To: histonet
Sent: Thu, Feb 23, 2012 3:08 pm
Subject: [Histonet] Paraffin Block Shipping Question
Hi,
am wanting to send out blocks to different site
I have also noticed that the temperature in the lab makes a big difference. Now
that it is colder outside and some heaters kick on check your temps.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brian f
I agree however; leaving blocks on the cold plate too long will also cause
cracking of blocks even at -2C.
- Original Message -
From: "Rene J Buesa"
To: histonet@lists.utsouthwestern.edu, "Brian foster"
Sent: Wednesday, January 4, 2012 11:50:13 AM
Su
Rapid cooling is the problem, but I do not think that -5ºC can cause it. The
effect is more noticeable in large but thin blocks.
René J.
--- On Wed, 1/4/12, Brian foster wrote:
From: Brian foster
Subject: [Histonet] Paraffin Blocks Cracking
To: histonet@lists.utsouthwestern.edu
Date: Wednesda
I always added melted paraffin from a paraffin dispenser into the hot chamber
of the embedding station.
It never crossed my mind to try to recycle paraffin. I strongly recommend a
cost study (cost of such instrument if there exists one) against cost of new
paraffin to replace "used"
(reagents an
As usual, Rene Buesa has a good sense of what might be worth changing - Do not
change anything to please a salesman.
"Change is inevitable. In a progressive country change is constant." (Benjamin
Disraeli, 1804-1887, cited from the Oxford Dictionary of Quotations). Dizzie
was Britain's firs
And are you surprised that the seller of the product includes glowing comments
from "users"?
If you are satisfied with the paraffin you are using now, why would you like to
change?
Change is not always better!
René J.
--- On Tue, 11/15/11, Alicia R. Lange wrote:
From: Alicia R. Lange
Subject
Our paraffin melts at 60C with pressure from a vacuum. I don't know if
there's actually enough pressure to affect the melting point of the
paraffin, but we always check the thermometer hanging inside the oven to
make sure it stays at 60C.
If you have a certain brand you're not sure of, I would che
water and you read the temperature at which the paraffin, contained in the
capillary , melts.
Best Regards,
Massimo Tosi
Da: Amber McKenzie
A: Jennifer MacDonald ; Zoe rosa
Cc: ""
Inviato: Lunedì 7 Novembre 2011 15:33
Oggetto: RE: [Histonet
:
Subject: Re: [Histonet] paraffin temperature
It depends on the paraffin. The melting point is usually on the bag.
Sent from my iPhone
On Nov 4, 2011, at 8:28 PM, Zoe rosa wrote:
>
> Hello all,
>
> I need help with something basic: What is the melting temperature for
> pa
It depends on the paraffin. The melting point is usually on the bag.
Sent from my iPhone
On Nov 4, 2011, at 8:28 PM, Zoe rosa wrote:
>
> Hello all,
>
> I need help with something basic: What is the melting temperature for
> paraffin?
>
> I appreciate your help,
>
> Thanks, Ismael
The brand of the paraffin has nothing to do with the melting temperature, which
depends on the paraffin polymers it contains.
Each paraffin will have a melting point value in the package and will melt at
that temperature. You can use a higher temperature also but not a lower one.
René J.
--- On
...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
[rjbu...@yahoo.com]
Sent: Saturday, July 23, 2011 10:46 AM
To: histonet@lists.utsouthwestern.edu; Joanne Clark
Subject: Re: [Histonet] Paraffin Wax Waste Disposal
I always incinerated my used paraffin
René J
--- On Fri, 7/22/11, Joanne Clark
I always incinerated my used paraffin
René J
--- On Fri, 7/22/11, Joanne Clark wrote:
From: Joanne Clark
Subject: [Histonet] Paraffin Wax Waste Disposal
To: "histonet@lists.utsouthwestern.edu"
Date: Friday, July 22, 2011, 4:22 PM
Hi All, we had our CAP inspection yesterday and were cited fo
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Wax Waste Disposal
That's funny that you got cited for that. I was surprised to learn what our
safety officer setup at my lab for paraffin disposal. A commercial company
takes our paraffin and makes a product with it that is mixed with cedar
sawdus
That's funny that you got cited for that. I was surprised to learn what our
safety officer setup at my lab for paraffin disposal. A commercial company
takes our paraffin and makes a product with it that is mixed with cedar
sawdust and paraffin wax for starting fires in the BBQ, fireplace, or
camp
Bret
We have done a bit of work with these types of samples, the first thing
we do is remove the formalin and add eosin to the container for about 5
minutes. Then we place the pellet (using a plastic disposable pipettor)
in a mesh bag and process on our tissue processor on a cycle that is set
at
@lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin blocks from cell line
we made a cell block from cell line by following procedure:
- centrifuge cell suspension
- fix the sediment overnight in formalin (for tissue fixation) - optional,
the button will be anyway fixed by subsequent procedure
we made a cell block from cell line by following procedure:
- centrifuge cell suspension
- fix the sediment overnight in formalin (for tissue fixation) - optional, the
button will be anyway fixed by subsequent procedure
- centrifuge and decant the formalin
- add few drops of liquid agar (not too
Each paraffin bag states the melting point temperature of the contained
paraffin. Just use that value +1ºC
René J.
From: "Artim, Kimberly"
To: "'histonet@lists.utsouthwestern.edu'"
Sent: Tuesday, May 17, 2011 11:57 AM
Subject: [Histonet] Paraffin temps
Could someone please share with me their
rsday, March 17, 2011 2:16:09 PM
Subject: RE: [ Histonet ] paraffin for pathology lab.
It sounds like you are looking for paraffin for infiltration (not embedding).
If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin
Type 1 - The same company makes Types 3, 6, and 9 but
It sounds like you are looking for paraffin for infiltration (not embedding).
If this is so, use a paraffin with shorter polymers like Richard-Allan Paraffin
Type 1 - The same company makes Types 3, 6, and 9 but these longer polymer
paraffins are designed for sectioning and ribbon formation.
As long as you order a quality paraffin from a reputable laboratory
supply company, you should be alright. I've used just about every paraffin
out there, and they are all adequate. Some are better than others, and
everyone has their personal preference. My personal favorite is Paraplast
Plu
Frozen sections are not good to study the histology.
Frozen are better for immediate diagnosis , enzyme study and special
staining like IHC- where formalin masked some of antigens.
Amita
From:
"Hehl Joachim"
To:
"histonet@lists.utsouthwestern.edu"
Date:
11/03/11 04:20 PM
Subject:
[Histonet]
Frozen sections (cryostat) are for when:
- there is a need for speed
- when fixing and processing tissue through to paraffin would
destroy/extract the component needed for diagnosis
Speed - such as when the patient is still on the operating table and a
diagnosis is needed immediately
Fixation/
I have always liked paraplast. It's good because you don't seem to have
as much tissue separation from the paraffin in the block. It also seems
to hold up longer on the water bath to give sections a chance to
"de-wrinkle" themselves without having to pull so much with forceps. It
also doesn't se
I use Gem Cut Paraffin, Pink Sapphire. I like the way it ribbons and I'm using
it in the processor and for embedding. I process/cut animal tissue. It comes in
other colors but we are all girls here so we chose pink.
Andi Grantham
On Jan 27, 2011, at 12:06 PM, Chiriboga, Luis wrote:
> HI all
I have never used Clear Rite or any other xylene substitute in my lab.
Part of the reason may involve something about teaching an old dog new
tricks. But there is another reason too. A few years ago I had to
paraffin embed and section tissues containing fibers of a new polymer
that was to be used
I had never used clear rite before, but in coming to this lab it is what
they use because they don't want that much xylene around. However, we
do still have xylene in the lab. I thought the problem you describe was
just a product of the clear rite so my solution...the first change after
the oven
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker
Sent: Wednesday, November 10, 2010 9:17 AM
To: Michael Mashore; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin Tissue Crumbles
Having some experience processing
Having some experience processing and embedding rodent tissues myself (by
hand), I would say that you are over-dehydrating the tissues. Try cutting
back the alcohol incubation times to 30 min or even 10 min each.
Regards,
Merced
--On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore
w
Sorry - short addenda - depending on what they were doing with this tissue
may also be critical as over processing or extended fixation may affect the
tissue microscopic evaluation.
Vikki
On Fri, Nov 5, 2010 at 7:37 AM, Victoria Baker wrote:
> Hi -
>
> Whole mouse livers do not need to be fixed
Hi -
Whole mouse livers do not need to be fixed for 72 hours given the process
you described. The protocol I followed for fixation of whole mouse organs
was within 24 hours, also when processing I started at 70% etoh, I also
included a 50/50 ratio of abs/xylene mix for my first clearing as it was
Thanks to all the prompt responses. He fixed the entire liver in excess
formalin (I think around 15 mL) with rocking for 3 days. I'm not sure about
the processing schedule; I'll have to contact the histology core to ask.
I forwarded all of the advice along, and he tried soaking the blocks in ice
w
Sounds to me like they are overprocessed (dried out). Try soaking them on
ice for 30 minutes or so before cutting. Some people use a drop of fabric
softener on their ice tray to soften the tissue, or there are commercial
products like soft block from Polyscience.
What is your processing schedule for the livers? It is hard to know without
that information.
Pam Marcum
UAMS
- Original Message -
From: "Adam ."
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, November 4, 2010 1:08:03 PM
Subject: [Histonet] Paraffin liver section
In Vermont we are very frugal. What I have used for more years than I care to
remember are pizza boxes from Pizza Hut. Our local Pizza Hut gives them to us
for free. They are very sturdy and perfectly fit 11 rows across and
approximately 36 blocks per row. I have 20 years of blocks stored th
We leave ours on all the time; we do shut off the cryo center each night (it
frosts up otherwise).
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Tuesday, May 18, 2010 2:41 PM
To: hist
I was instructed by a very knowledgeable person in the field that the
best processing for your tissue is to change it as often as you change
your clearant. So, if you change your clearant after 5 uses, you should
also change the parafffin after 5 uses too.
Hope this helped,
Lynette
Lynette Pavel
You cannot "measure" paraffin changes by dates because it is used as a function
of the number of cassettes processed. I always used VIP that have 4 paraffin
containers. I used to keep track of the number of cassettes processed daily.
When I got to as many cassettes as the VIP was designed to (e.
I have my processor "set" to notify me at 1500 blocks, at which time I
change the paraffins. I usually run 500 in the other solutions (same
notification system) before changing. It works for me!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerqu
This will depend on the amount of blocks processed.
I worked in places who used to change their VIP twice a week on Wednesday &
Friday, and other that would change processor solutions once a week, but as a
rule, processor wax regardless of the make should ALWAYS be changed after a
maximum of 5
I have noticed that most slips happen in the junctions between the
paraffin covered floors and " uncoated" floors, such as doorways to
outside halls. The change in traction does it. Also, hard soled shoes are
fine, as long as they have a build up of paraffin on them. If a visitor (or
patho
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin Accumulation on floor
We have a long-handled scraper and use it periodically.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun
We also use the long-handled scraper. Our greatest problem though, was the
hallway outside of Histology where non-technical staff would be walking in
heels. We eventually had that hall carpeted, which has been a great improvement.
-Original Message-
From: histonet-boun...@lists.utsouthw
We have a long-handled scraper and use it periodically.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
A good tech will know how to cut good, wrinkle free sections. Making a good
section is technique making the ribbon, transferring on the water bath, letting
the tissue expand... when to pick it up, when to leave it on the water longer,
etc. Some tissues take longer to become wrinkle free, such
: 'Scott, Allison D'
Subject: RE: [Histonet] Paraffin Disposal
Once it is hardened, I put it in the regular waste (haven't had any questions
raised about that practice). Would be interested in other responses.
Peggy
-Original Message-
From: histonet-boun...@lists.uts
It is incinerated.
René J.
--- On Mon, 1/11/10, Scott, Allison D wrote:
From: Scott, Allison D
Subject: [Histonet] Paraffin Disposal
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 1:24 PM
Happy New Year to all. How are you all disposing the paraffin that
comes off of
Wipe over the microtome and the immediate area with a dryer sheet.
Jackie O'
From:
"Sebree Linda A"
To:
"Emily Sours" ,
Date:
10/01/2009 02:21 PM
Subject:
RE: [Histonet] paraffin sectioning static
Sent by:
histonet-boun...@lists.utsouthwestern.edu
The thi
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