RE: [Histonet] Paraffin blocks from cell line

2011-05-18 Thread Bernice Frederick
@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin blocks from cell line we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure

RE: [Histonet] Paraffin blocks from cell line

2011-05-18 Thread IRENA SREBOTNIK KIRBIS
we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too