I believe you are describing the procedure for Michel's Fixative Solution. Janet
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Michelle MacVeigh-Aloni Sent: Mon 8/3/2009 7:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: from PLEASE HELP Hi Delia, Some time ago I used to work in a lab where I had to cut skin punch biopsies. They used to come floating in a special preservative. Unfortunately I don't remember its name, but then I had to wash them in a special wash solution for a minute. The wash solution was made by Zeus Scientific and was just citrate buffer. I am not familiar with the Michael's fixative and don't know do you have to wash after fixing. The trick was to pat dry the biopsies with Kimwipes and immerse them right away in the OCT. Then let the biopsy sit there for few minutes. You can even move the tissue in the OCT from time to time and then transfer it to a new mold with fresh OCT. Freeze in liquid Nitrogen. This way, somehow the OCT starts infiltrating the tissue and the tissue cuts together with the OCT. I used to cut it at 5 microns. Hope that this will work for you too Michelle Research Specialist USC Keck School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
