Date: 07/07/11 07:10 PM
Subject: RE: [Histonet] Routine H & E stain,
I have always used Harris and have not experienced this problem? I do
buy mine pre-made from VWR (cat#-95057-858). I agree with the xylene
comment, also what mounting media are you using? The mounting media can
@lists.utsouthwestern.edu; histonet;
amitapan...@torrentpharma.com
Subject: Re: [Histonet] Routine H & E stain,
It seems that your mounting medium is acid. Try to correct that rather than
changing the staining.
René J.
--- On Thu, 7/7/11, amitapan...@torrentpharma.com
wrote:
From: amitapan.
It seems that your mounting medium is acid. Try to correct that rather than
changing the staining.
René J.
--- On Thu, 7/7/11, amitapan...@torrentpharma.com
wrote:
From: amitapan...@torrentpharma.com
Subject: [Histonet] Routine H & E stain,
To: "Histonet" ,
histonet-boun...@lists.utsouthwes
Amita,
We are a neurotoxicology lab that also provides histology services for
other researchers inside & outside Rutgers, and we use Gill's #3
hematoxylin for our routine H&Es on mouse and rat tissues. No fading that
I have noticed in all the years that I have been here. We get ours from
Thermo
I should also have added not all synthetic Xylenes do this and I also use a
Microwave Processor. These variables may have been a factor. Wonderful world of
Histology, TRIAL AND ERROR.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, C
Are you using synthetic xylene in your H&E/COVER SLIPPING protocol. If yes, you
might want to consider ending your protocol with the real thing (XYLENE). I had
the same situation until we switched to real XYLENE. No more H&E fading.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY
