boun...@lists.utsouthwestern.edu [mailto:
>> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood
>> (SCHN)
>> Sent: Tuesday, 24 July 2012 9:00 AM
>> To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Secondary an
9845 3318
> >> the children's hospital at westmead
> >> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> >> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> >>
> >>
> >> -Original Message-----
> >> From: histonet-boun...@lis
__
> From: histonet-boun...@lists.utsouthwestern.edu [
> histonet-boun...@lists.utsouthwestern.edu] on behalf of Eva Permaul [
> e...@georgetown.edu]
> Sent: Tuesday, July 24, 2012 8:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histo
istonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Secondary antibody causing nuclear staining?
>
> I understand the point about the biotin and I should have said that when
> using the ABC method we have taken to always using an avidin/biotin
> blocking kit. We are using biotiny
outhwestern.edu] On Behalf Of Tony Henwood
>> (SCHN)
>> Sent: Tuesday, 24 July 2012 9:00 AM
>> To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Secondary antibody causing nuclear staining?
>>
>> It is possible that this
Of Tony Henwood
> (SCHN)
> Sent: Tuesday, 24 July 2012 9:00 AM
> To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Secondary antibody causing nuclear staining?
>
> It is possible that this is due to "Biotin nuclei" where excess bi
od
(SCHN)
Sent: Tuesday, 24 July 2012 9:00 AM
To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Secondary antibody causing nuclear staining?
It is possible that this is due to "Biotin nuclei" where excess biotin is found
in the nuclei of some cells, see b
It is possible that this is due to "Biotin nuclei" where excess biotin is found
in the nuclei of some cells, see below:
Optically clear nuclei have been reported in endometrial epithelium associated
with first and second trimester abortions (Sickel & di Sant'Agnese 1994).
Optically clear nuclei
We believe it has to be the secondary as we have nuclear staining in the
absence of primary antibody.
Eva
On Mon, Jul 23, 2012 at 12:58 PM, ihcs-wojcieszyn wrote:
> Hello,
> Please explain how you know that it is the secondary and not the
> primary? We Have confirmed that in canine lymph
