RE: [Histonet] Stain for osteoporotic bone in Spurr plastic

2012-11-09 Thread Patsy Ruegg
: RE: [Histonet] Stain for osteoporotic bone in Spurr plastic Allison, I am not exactly certain if this protocol will work for Spurr's embedded specimens, but here is the protocol that I use for deplasticized sections of methyl methacrylate embedded undemineralized bone (non-decalcified): Xy

RE: [Histonet] Stain for osteoporotic bone in Spurr plastic

2012-11-08 Thread Jack Ratliff
Allison, I am not exactly certain if this protocol will work for Spurr's embedded specimens, but here is the protocol that I use for deplasticized sections of methyl methacrylate embedded undemineralized bone (non-decalcified): Xylenes - warmed @ 50 C for 30 min w/ dip & dunk @ 15 min interval Xy