ay 01, 2015 4:27 PM
To: James Watson; 'Jb'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:
With Mount Quick you can also decolorize the section and do a different stain
on them. I have even performed IHC on these transferred sections when needed.
27;Jb'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue transfer method:
Slide Repair
1. Soak coverslip off of slide in xylene.
2. Piece slide back together.
3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4. Place slide in cold water in re
Slide Repair
1. Soak coverslip off of slide in xylene.
2. Piece slide back together.
3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight.
4. Place slide in cold water in refrigerator overnight.
5. Carefully peal the Mount-Quick off the slide. The tissue
boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, April 13, 2011 3:54 PM
To: histonet@lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett
Subject: Re: [Histonet] Tissue transfer
"Mission impossible" (successfully, you can always ruin them)!
It is better to d
"Mission impossible" (successfully, you can always ruin them)!
It is better to do the staining after a prolonged oven heating and do it
delicately.
René J.
--- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) wrote:
From: Bartlett, Jeanine (CDC/OID/NCEZID)
Subject: [Histonet] Tissue trans
I use Harleco Krystalon liquid coverslip medium. Cover the tissue, laying
horizontally in oven for a few hours until it hardens, than soak in water
bath, peel, and transfer to charged slide with same side facing down, dry
in oven again, dissolve in xylene when ready for staining.
Diane Craft
P
