RE: [Histonet] Trouble making a good Cell Block

2009-08-26 Thread Monfils, Paul
To make a cell block from formalin-fixed cells, you need to introduce some coagulable material that will stick the cells together. Here's a method I have used successfully: Spin down the cells in formalin, decant as much formalin as possible with a pipette. Fill tube with isotonic buffer, resu

Re: [Histonet] Trouble making a good Cell Block

2009-08-25 Thread DKBoyd
Hi Maria, Are you saying you are using formalin as a preservative for non-gyn's? We collect all aspirations in Plasmalyte which is an electrolytic balance for infusion. All other fluids are collected in a green top, sodium heparinized tube. Bronch specimens are sent in saline. Urine and CSF a