17 PM
To: Smith Wanda; Anne C Lewin; Eva Permaul
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] bluing in tap water?
Is there such a thing as bluing too much?
-Original Message-
From: Smith Wanda
Date: Mon, 12 Jan 2009 15:12:12
To: Anne C Lewin; Eva Perma
Is there such a thing as bluing too much?
-Original Message-
From: Smith Wanda
Date: Mon, 12 Jan 2009 15:12:12
To: Anne C Lewin; Eva Permaul
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] bluing in tap water?
What should the pH of tap water be to blue just right and
ts.utsouthwestern.edu] On Behalf Of Smith
Wanda
Sent: Tuesday, 13 January 2009 8:12 AM
To: Anne C Lewin; Eva Permaul
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] bluing in tap water?
What should the pH of tap water be to blue just right and not too
much???
WANDA G. SMITH,
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne C Lewin
Sent: Monday, January 12, 2009 12:04 PM
To: Eva Permaul
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] bluing in tap water?
When I have used tap water, I use cold running water for 5 minutes.
Works
When I have used tap water, I use cold running water for 5 minutes.
Works fairly well, depending on the pH of your tap water.
Eva Permaul wrote:
Good morning,
I was wondering if someone uses tap water to blue their slides after
Hematoxyline. If yes, do you use warm or cold water and for how
Bluing in tap water is quite often done but has the disadvantage that your
protocol cannot be repeated in other Lab because not all tap waters have the
same composition of "bluing
quality".
As any other chemical reaction it is better to blue in warm tap water (it will
take less time) but you ca
Hello,
I rinse in tap water, but blue in 1-2% ammonium hydroxide for ~ minute, then
rinse again in tap water.
Paula
From: Eva Permaul
To: histonet@lists.utsouthwestern.edu
Sent: Monday, January 12, 2009 8:35:15 AM
Subject: [Histonet] bluing in tap water?
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