>
>
> My experience with anti-roll plates is mostly with the old International
> cryostats of days gone by. Pathologists usually disdained anti-roll plates
> and used an artist's brush to keep the section from rolling, but more
> proficient users depended on them. I learned to use them in a researc
insky
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate
Maybe there's a product that increases friction with screw threads, doesn't
have toxic outgassing, and works at low temperatures.
I'm going to look for something like this.
Geri
-Original Mes
8:32 AM
To: Ken Marzinsky ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate
WARNING: This email appears to have originated outside of the UW Health email
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know
the content is safe.
We ha
We have 3 new leicas and we constantly have to adjust them. They creep forward
so much that I just automatically know to roll it slightly back after every 4
specimens or so. The new leica designs are just bad and customer service is
even worse. We have to use the plate as we do Mohs specimens an
I use roll plate.
I don't like the roll plate on the Leica. The Leica design creeps... a lot.
Also, with the leica, the contact point with the blade is a soft aluminum arm.
If you make adjustments with the roll plate holder contacting the blade, it
will damage the soft aluminum holder.
I li
Tony - We were on the same page. We purchased fresh beef liver, sectioned and
froze it, cut and stained duplicate blocks and had the pathologist sign off on
the process. We were also able to get some tonsils delivered fresh and did
them, too. Terri
Terri L. Braud, HT(ASCP)
HNL Laboratories f
ll,
> Mehndi Helgren
> Dominion Pathology Laboratories
> 733 Boush Street
> Suite 200
> Norfolk, VA 23510
> 757-664-7901
>
>
>
> ----------
>
> Message: 2
> Date: Mon, 9 Aug 2021 13:56:42 +
> From: "Morken, Timothy"
> To: Histology
> Cc: Histon
Norfolk, VA 23510
757-664-7901
--
Message: 2
Date: Mon, 9 Aug 2021 13:56:42 +
From: "Morken, Timothy"
To: Histology
Cc: Histonet
Subject: Re: [Histonet] cryostat
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hi Mehndi,
Same here, I had been using Epredia cryostat for the last several years. I
like it very much. A video on youtube shows its beautiful features.
https://youtu.be/oMNt6RT5cWI
Best,
Haiyan
On Mon, Aug 9, 2021 at 7:04 AM Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wr
Mehndi, we also cut frozens for DIF on kidney and muscle histochem. We have two
Epredia NX70 cryostats. We've used them for 8 years now and really like them.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Or
Hi Brittany,
Personally I do not like the HM525NX. It is awkward and difficult to control
with no manual advance except the control panel. When advancing /trimming you
are never certain how far forward the block will travel. The UV light fitting
in the lid always seems to get in the way of my
-Original Message-
From: Morken, Timothy via Histonet
Sent: Wednesday, May 19, 2021 8:26 AM
To: Brittany Hethcox
Cc: Histonet
Subject: Re: [Histonet] Cryostat
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're
reliable and easy to use. We have the elevator on ours a
Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're
reliable and easy to use. We have the elevator on ours and it makes a
difference for different size people in the lab. It also can have a vapor
disinfectant system which we use and it works great.
We use ours for kidney
The amount of time a cryostat takes to get to cutting temperature depends on
the age of the machine, manufacturer, and air flow around the cryostat. We have
4 Avantiks the newer ones cool faster than the older ones and the one that is
under a wall cabinet with about 2 feet from the top of the cr
Our last discussion with TJC to clarify their definition of "regular interval"
was to follow the manufacturers guidelines. I recommend determining what
manufacturer suggests with the use of the UV decon and write that in your
policy that addresses this standard. If you are following their guid
Gary
I think that might have to do with the fan motor inside the unit, it might need
to be replaced. We had ours replaced a few years ago. Do you service/PM the
cryostat yearly?
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-394
: Friday, March 11, 2016 12:52 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Cryostat with UV disinfection
For those of you that are CAP accredited, and use a cryostat with UV
disinfection, how do you get around the CAP requirement listed below? We were
told the UV di
For those of you that are CAP accredited, and use a cryostat with UV
disinfection, how do you get around the CAP requirement listed below? We were
told the UV disinfection did not replace wiping down with a tuberculocidal
disinfectant at room temp. Do you have an approved tuberculocidal disinfec
Hello Patrick,
We have this same cryostat except it's the CM 3050S. Ours also has
this issue usually after it's been running for many months with a
defrost. The typical cause of your problem is the cylinder on which
the block holder is attached has ice crystals or is "frozen". I feel
the cause
Dear Kim,
Yes the Starlet cryostat made by Bright Instrument Company, it is in a small
bench level cabinet as there is no other way of achieving good results on the
refrigeration. Apart from freezing microtones which were highly inefficient on
retrieving good quality thin sections.
Hope this as
cold cryostat with 100% alcohol.
Hugh
Hawaii
--
Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST)
From: Madeline Gi
Subject: Re: [Histonet] Cryostat
To: "Abbott, Tanya" ,
"Histonet@lists.utsouthwestern.edu"
Message-ID:
<1389897789.16602.yahoomai
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it
clean it.
best of luck.
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com
On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya"
wrote:
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat service
Avantik is located in NJ and services cryostats in addition to microtomes,
processors and stainers.
www.avantik-us.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
Avantik is located in NJ and services cryostats in addition to microtomes,
processors and stainers.
www.avantik-us.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Ross
Sent: Monday, October 28, 2
Hello,
BelAir is an excellent repair service company located in NJ. They will come to
Delaware for repairs. Look them up online for the contact information.
Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-33
We wipe out the excess tisue( if there is any), wipe down the chamber with
abs. Alcohol then spray the chamber with "CaviCide", let it sit for @ 3-5
minutes and then wipe the chamber out again.
Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washin
Ann,
There are many tuberculocidal cleaners. Just search for it. If you are in a
hospital the central supply will most likely have some in stock.
We use PD-128 by Spartan Chemical
http://www.spartanchemical.com/products/product/101604
Here are some references for cryostat cleaning:
Cryos
Hi,
Leica 1850s are very reliable, 1860 is basically the same unit with very
precise electronic specimen feed.
UV lamp's lifetime is about 8k hours, and every switch on cycle adds
another hour to the counter. New lamp will cost you a few hundred euro,
though. Any operator can replace the lamp, no
Ann,
Cryostat decontamination references:
College of American Pathologists, Commentary on certification questionaire:
1) Decontaminate regularly with 70% alcohol.
2) Defrost, remove trimmings and decontaminate with a tuberculocidal
disinfectant, preferably weekly for instruments use
Hi Irena!
If you go to IMEB Inc.'s website, and type "vacuum" into the search, it will
take you to what we use. They sell a vacuum cleaner and accessories. We
currently use the filtered hoses with an old canister vacuum that's been around
longer than I have. When the filter gets full, we dis
-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Bright
[abri...@brightinstruments.com]
Sent: Wednesday, February 06, 2013 9:31 AM
To: IRENA SREBOTNIK KIRBIS
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat
I would not vacuum the trimmings out of cryostat, if it were
I would not vacuum the trimmings out of cryostat, if it were infected It would
make a good job of contaminating a laboratory etc.
Terrified Alan Bright
Sent from my iPhone
>
>
>
>
> Hi,I wonder what is the way of removing shavings/trimmings from the cryostat
> in other lab?, with the wet p
Hi Eric,
Mikron Instruments services most of our instruments, including Micron
brand. I don't have any Leica equipment now, but they have serviced my
Leica microtome in the past. They are very good! The service manager's
name is Steve Pike. Mikron is in Vista, CA and their phone number is
(800
@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat repair--thanks!
The compressor fan was loose, so when the Tech One guy fixed it, instead of
making a horrible noise when it turns off, it just rattles. Oh well, time for
a new motor.
Which leads me to another question, is there any reason I should call Lecia
The compressor fan was loose, so when the Tech One guy fixed it, instead of
making a horrible noise when it turns off, it just rattles. Oh well, time
for a new motor.
Which leads me to another question, is there any reason I should call Lecia
to replace this motor? The guy from Tech One Biomedical
...@lists.utsouthwestern.edu] On Behalf Of DANIEL
HEWITT
Sent: Thursday, March 31, 2011 2:15 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair
Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and
do most of our repairs. Ray does an excellent job at a
DANIEL
HEWITT
Sent: Thursday, March 31, 2011 2:15 PM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat repair
Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds
Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.
Daniel Hewitt
Histology Supervisor, HVS
412-749-7371
This email, including any attac
Hi Diane,
We just purchased a cryostat from Sakura, we love it. Great features.
> Date: Wed, 30 Mar 2011 06:38:41 -0700
> From: dch...@yahoo.com
> To: diane.glad...@us.army.mil; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Cryostat Feedback Needed (UNCLASSIFIED)
&g
Hi Diane,
We have been happy with the Leica cryostats over the years. We have an 1800
that is about 15 years old, an 1850 that is about 5 years old with the UV light
for decontamination.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
__
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, January 11, 2011 10:29 AM
To: histonet@lists.utsouthwestern.edu; KimberlyBlundon
Subject: Re: [Histonet] Cryostat help
That depends on what staining you need to use.
If an IHC, just fi
That depends on what staining you need to use.
If an IHC, just fix the sections in acetone, air dry and proceed without HIER
If H&E fix quickly with formalin 10%, wash → hematoxylin for 30 secs without
differentiation → eosin 15 sec→ dehydrate and mount.
Other HC procedures all will require fixati
27;Donnell, Bill
Sent: Wednesday, November 24, 2010 4:14 PM
To: Paula Lucas; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cryostat Question Please
Very consistant, easy to use.
2 drawbacks
1. We got the decontamination add-on trying to automate the CAP
requirement, but now it se
Very consistant, easy to use.
2 drawbacks
1. We got the decontamination add-on trying to automate the CAP
requirement, but now it seems Thermo says it is not an adequate process.
I believe they are doing more intense studies, but for the time being, I
have to decontaminate it by hand.
2. OCT
Dear Susan,
Our Starlet 2212 Bench Top Cryostat has an option for orientation. There are
many used for Mohs, as holding a low temperature is not a problem. I can email
you some user references if. You want.
Regards: Alan Bright
Bright Instrument Co. Ltd.
England
Sent from my BlackBerry® wireles
: 'Jeffrey Thompson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat
Jeff,
We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us. You can find out more
information on the unit at the following URL:
http:/
Jeff,
We have both the Leica CM1850 and the CM1850 UV. These are not top of the
line models, but they work very well for us. You can find out more
information on the unit at the following URL:
http://www.leica-microsystems.com/products/histology-systems/sectioning/cryo
stats/details/product/leic
It may help to let your block equilibrate for about 20 minutes in the
cryostat. I've found blocks do not section well if you start sectioning
right away, especially snap frozen ones. Also, do you embed in OCT/sucrose?
A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever
embed
Most definitely there are differences.
Just consider that the bone will not be subjected to any chemicals because
neither fixation nor demineralization will be required.,
René J.
--- On Mon, 5/10/10, louise renton wrote:
From: louise renton
Subject: [Histonet] Cryostat vs decal sections of bo
Dear Mohamed,
We manufacture cryostats but not the one you have the problem with, however I
think what you can hear is the cooling fan working but not the refrigeration
compressor.
You will need to locate the internal fuses to see which ones need replacing. If
the compressor is running then it
I agree with Joyce, it sounds like the fuse for the refrigeration. I had that
problem a few times.
Robyn Vazquez
> Date: Sun, 21 Mar 2010 08:28:36 -0400
> From: jwe...@sjha.org
> To: k8...@yahoo.com; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] cryostat help
our cryostat is
Minotome Plus
Microtome Cryostat
Cat. No. 2563-- For 120 VAC, 60Hz
--- On Sat, 3/20/10, Cheryl Cornett-Early wrote:
From: Cheryl Cornett-Early
Subject: Re: [Histonet] cryostat help please!
To: "mohamed abd el razik"
Date: Saturday, March 20, 2010, 10:59 PM
Sounds like it may be a fuse... in my non-expert opinion!! j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu on behalf of moh
thanks for your replay ray
yes i set the temp. to -20 degrees but it is 20 for the last week.
--- On Sat, 3/20/10, Mark Ray wrote:
From: Mark Ray
Subject: Re: [Histonet] cryostat help please!
To: "mohamed abd el razik"
Date: Saturday, March 20, 2010, 5:03 PM
Are you sure the t
We currently have a Leica and a Jung. They are serviced twice a year. Prior to
these, we had two Tissue Teks that were donated to us. My best guess is that
they were about 20 years old when they were replaced.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
for repair.
Thanks,
Pam Marcum
UAMS
Anatomic Patholgoy Manager
- Original Message -
From: "Tim Morken"
To: "Jeffrey Silverman" , "o isaac24"
Cc: histonet@lists.utsouthwestern.edu
Sent: Monday, February 22, 2010 10:23:17 AM GMT -06:00 US/Canada C
Jeff, Do they give any references for the effectiveness of their proposed
method?
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On B
...@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham
Sent: Tuesday, December 01, 2009 2:22 PM
To: Merced M Leiker
Cc: Histonet
Subject: Re: [Histonet] Cryostat Poll
yes and yes
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box
yes and yes
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097
"happy slicing and dicing and may all your stains
>From what I understand if you require that an individual wear a N95
respirator when they are sectioning frozen sections then you are also
required to have that respirator fit tested yearly.
We are a research lab that does quite a bit of work on TB samples. We
do not section TB infected samples
How about to use a drop of water?
Sometimes the section is a bit big, and it is hard to mount the section very
flat on slides without a bubble...
2009-04-20
TF
发件人： Paula Pierce
发送时间： 2009-04-03 23:59:43
收件人： tifei
抄送：
主题： Re: [Histonet] Cryostat sections mounting with 0.1M PB
We have the adapter here at IMEB, Inc.
Please go to www.imebinc.com for
our contact info and ask for Brad.
Thank you
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
southwestern.edu] On Behalf Of Ingles Claire
Sent: Wednesday, January 07, 2009 7:38 PM
To: Histonet
Cc: mari.ann.mailh...@leica-microsystems.com
Subject: RE: [Histonet] Cryostat safety question
Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to
say, we don
Message--
From: Alan Bright
To: Andrea Hooper
To: Histonet
Sent: Jan 8, 2009 7:06 AM
Subject: RE: [Histonet] Cryostat safety question
Andrea,
For users of our cryostats that need to use the specimen for future use
we manufacture disposable object holders & quick release holders that
fits spec
ginal Message-
From: Ingles Claire
Date: Wed, 07 Jan 2009 18:38:04
To: Histonet
Cc:
Subject: RE: [Histonet] Cryostat safety question
Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to
say, we don't have 50 chucks laying around... In the morning before clin
Andrea,
For users of our cryostats that need to use the specimen for future use
we manufacture disposable object holders & quick release holders that
fits specimen containers for LN2 or -80 deg. C storage. The tissue stays
on the disposable object holders during storage too and has an index
locat
I have the same cyostat, but it doesn't matter which one you use for
removing tissue from the chuck is a separate issue. Others may beg to
differ from my technic, but I remove the block from the cryostat and set it
on a paper towel for a couple of mintes. I do not thaw tissue, just soften
it. I ta
Andrea:
I work in a Mohs clinic where all we cut is frozen skin sections. Needless to
say, we don't have 50 chucks laying around... In the morning before clinic
starts we put a layer of freezing medium on chucks and put them in the cryostat
to freeze. When we get specimens, we add another drop o
Hi Andrea,
It's great to hear that you are happy with your cryostat and, hopefully,
I'll be able to help you with an answer to your question. Leica sells a
small device called a Thermal Block that is designed to help you remove the
specimen from the chuck without causing it to melt. The catalog
The Microm HM 550 is an awesome cryostat, and meets requirements 1-3 for
sure. Not sure about the price. I think we got ours used... There is room
for a 100 count slide box, but you'd have to maneuver it a bit in order to
get the lid open and a slide in there.
William Connor
-Original Mess
As part of the general SOP, under sectioning.
René J.
--- On Tue, 10/28/08, Michele Wich <[EMAIL PROTECTED]> wrote:
From: Michele Wich <[EMAIL PROTECTED]>
Subject: [Histonet] cryostat SOP
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 28, 2008, 3:46 PM
An SOP question for those out
Good going Only us OLD TECH know this.
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Weems,
Joyce
Sent: Wednesday, October 15, 2008 9:29 AM
To: Emily Sours; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cryostat blues
Those who are
histonet-bounces@ cc
lists.utsouthwest
ern.edu Subject
Re:
31 - Fax
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Emily
Sours
Sent: Wednesday, October 15, 2008 8:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] cryostat blues
Unfortunately, this sounds like static electricity.
Leica suggests
Unfortunately, this sounds like static electricity.
Leica suggests spraying anti-static spray in the chamber (!). You could
also ground the metal in the chamber using metal wire touching something
metal outside the chamber (the Russian post-doc solution, very clever). We
keep 100% EtOH in a small
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