n.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount
Sent: Monday, January 31, 2011 4:01 AM
To: Jan Berry; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] fixation question for IHC
It is possible to freeze paraformaldehyde in aliquots, so you don't have
It is possible to freeze paraformaldehyde in aliquots, so you don't have
to keep on making it up repeatedly as we did in the old days. I have
also seen that you can buy Pure formaldehyde, but I don't remember who
the supplier was. It was in sealed ampoules and was 16% so you could
dilute it in your
Jan,
Depends on your material, further processing and antigens. Surprisingly, some
antigens are better preserved after formaldehyde fixation than after buffered
formalin (probably, due to high sensitivity of particular epitope to alcohol
traces from standard formalin solution). I know at least o