RE: [Histonet] fixation question for IHC

2011-01-31 Thread Sherwood, Margaret
n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaret Blount Sent: Monday, January 31, 2011 4:01 AM To: Jan Berry; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] fixation question for IHC It is possible to freeze paraformaldehyde in aliquots, so you don't have

RE: [Histonet] fixation question for IHC

2011-01-31 Thread Margaret Blount
It is possible to freeze paraformaldehyde in aliquots, so you don't have to keep on making it up repeatedly as we did in the old days. I have also seen that you can buy Pure formaldehyde, but I don't remember who the supplier was. It was in sealed ampoules and was 16% so you could dilute it in your

RE: [Histonet] fixation question for IHC

2011-01-28 Thread Anatoli Gleiberman
Jan, Depends on your material, further processing and antigens. Surprisingly, some antigens are better preserved after formaldehyde fixation than after buffered formalin (probably, due to high sensitivity of particular epitope to alcohol traces from standard formalin solution). I know at least o