I will 2nd this.
When I did neuropathology at a major institution, we froze all frozen sections
in an isopentane slurry cooled with LN2. We waited for the OCT to warm to
cryostate temps before cutting. If there was time pressure from the surgeons, I
used my thumb to warm more quickly, until se
There is an OCT for lower temperatures.
- Original Message -
From: "Della Speranza, Vinnie"
To: "Bruce W Brodersen" ,
histo...@pathology.swmed.edu
Sent: Monday, August 9, 2010 9:40:03 AM
Subject: RE: [Histonet] friable or crumbly O.C.T.
I'm guessing that l
I'm guessing that liquid nitrogen or dry ice temperature is too cold for
sectioning OCT.
OCT cuts well down to about -25 degrees C.
Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp
range at LN2
You will want to give the OCT blocks the opportunity to "warm up" to cr
