RE: [Histonet] fungal stain for frozen sections

2013-05-16 Thread Tony Henwood (SCHN)
The Sulphation Toluidine Blue stain should work a treat and very quick to do: Sulphation - Toluidine Blue Technique for Fungi Following sulphation, fungi stain metachromatically with toluidine blue. Certain hydroxyl groups are esterified by sulphuric acid to form ester sulphate groups that sta

Re: [Histonet] fungal stain for frozen sections

2013-05-16 Thread robert_schoonhoven
At what wave length?  Eosin  is is also fluorescent  Sent from my Boost Mobile phone.    -- Original Message --From: Will Chappells Date: 5/16/2013 12:18 PMTo: wrg...@aol.com;Cc: histonet@lists.utsouthwestern.edu;Subject: Re: [Histonet] fungal stain for frozen sections It appears

Re: [Histonet] fungal stain for frozen sections

2013-05-16 Thread Will Chappell
It appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. I haven't performed it, however it could be as quick as an H&E stain. Looks promising. Http://rd.springer.com/article/10.1007%2FBF02889994#page-1 Wil