RE: [Histonet] mouse perfusion rate

2010-04-02 Thread Charles . Scouten
To: "Andrea T. Hooper" ; saby_josep...@yahoo.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu; charles.scou...@leica-microsystems.com Subject: RE: [Histonet] mouse perfusion rate Someone I believe on Histonet a couple years ago told me the same thing. The "perfusion circuit" i

RE: [Histonet] mouse perfusion rate

2010-04-01 Thread Merced M Leiker
..@leica-microsystems.com Subject: RE: [Histonet] mouse perfusion rate To: lei...@buffalo.edu, saby_josep...@yahoo.com, mak...@ufl.edu, histonet@lists.utsouthwestern.edu Date: Monday, March 29, 2010, 6:26 PM I have perfused mice and rats at 300 mm Hg, about double physiological level, don't kno

RE: [Histonet] mouse perfusion rate

2010-03-31 Thread Andrea T. Hooper
From: charles.scou...@leica-microsystems.com Subject: RE: [Histonet] mouse perfusion rate To: lei...@buffalo.edu, saby_josep...@yahoo.com, mak...@ufl.edu, histonet@lists.utsouthwestern.edu Date: Monday, March 29, 2010, 6:26 PM I have perfused mice and rats at 300 mm Hg, about double physiological

RE: [Histonet] mouse perfusion rate

2010-03-29 Thread Charles . Scouten
Monday, March 29, 2010 9:05 AM To: Joseph Saby ; charles.scou...@leica-microsystems.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate Hi Joe, Thanks for that notice about flow rates. But I think for the mouse you meant 1-3mls/min (not per 10m

Re: [Histonet] mouse perfusion rate

2010-03-29 Thread Merced M Leiker
; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Sent: Fri, March 19, 2010 9:21:38 AM Subject: RE: [Histonet] mouse perfusion rate The vasculature will leak too much and the mouse will get bloated - you'll see it first in either the intestines blowing up like a balloon or fluid coming out o

Re: [Histonet] mouse perfusion rate

2010-03-27 Thread Joseph Saby
From: Merced M Leiker To: charles.scou...@leica-microsystems.com; mak...@ufl.edu; histonet@lists.utsouthwestern.edu Sent: Fri, March 19, 2010 9:21:38 AM Subject: RE: [Histonet] mouse perfusion rate The vasculature will leak too much and the mouse will get bloated - you'll see it

RE: [Histonet] mouse perfusion rate

2010-03-19 Thread Merced M Leiker
8 PM To: MKing ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mouse perfusion rate That may be mouse cardiac output, but I can assure you, from experience, you do not want to perfuse at 17ml/min. Regards, Merced --On Thursday, March 18, 2010 1:32 PM -0400 MKing < mak...@ufl.edu&

Re: [Histonet] mouse perfusion rate

2010-03-18 Thread Merced M Leiker
That may be mouse cardiac output, but I can assure you, from experience, you do not want to perfuse at 17ml/min. Regards, Merced --On Thursday, March 18, 2010 1:32 PM -0400 MKing wrote: Li, Mouse cardiac output seems to be about 17 ml/min (e.g. www.transonic.com/mice1.shtml), you probably w