NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff
McAuliffe
Sent: Thursday, 23 September 2010 11:59 PM
To: Salim Yalcin Inan
Cc: Histonet
Subject: Re: [Histonet] post-fixation question
Hi Inan,
2h post-fixation for immunohistochemistry on mouse brain is usually more
than enough. We use 1h post-fixation at room temp for mouse brain
following storage (sometimes, for many weeks) at +4 C in PBS before
sucrose cryo-protection, embedding and sectioning.
Anatoli Gleiberman, PhD
Directo
Merced is correct about longer fixation inducing more cross-linking,
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my experience.
Geoff
Salim Yalcin Inan wrote:
Hello,
I have a q
The longer the tissue sits in paraformaldehyde the more the proteins in the
tissues will become cross-linked due to multiple methylene bridge
formations and could require very rigorous antigen retrieval or may even be
beyond retrieval; the tissues could become brittle as well due to
over-fixati
