Definitely go with Oil Red O rather than Osmium.
Osmium tetroxide reacts with unsaturated lipids whereas ORO colours neutral
lipids.
You can do an ORO on formalin fixed tissue (frozen sections).
You could also look at the Sudan Black techniques.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysA
Michelle
We use osmium all of the time, we find it works better for image
analysis than the Oil Red O.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
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