Silly me: http://stainsfile.info/StainsFile/stain/hematoxylin/h-and-e-eo.htm
Bryan
Bryan Llewellyn wrote:
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here i
Read this page about eosin counterstaining on StainsFile.
Bryan Llewellyn
Cesar Francisco Romero wrote:
I think that the nearest to what you wish is Eosin
- Phloxine Stain.
Here is the web page where you can find the formula.
http://protocolsonline.com/histology/dyes-and-stains/haematoxy
Wanda makes an excellent point, thinner sections have less contrast.
Geoff
On 8/9/2012 2:50 PM, wanda.sm...@hcahealthcare.com wrote:
How about the thickness of the tissue? Once we were cutting skins at 3 microns
and the pathologist complained the Eosin was too light so we started cutting
the
We have our grossing staff cover all GI biopsies, breast biopsies and
endometrial curettings or any small colorless tissue with 0.1% basic
fuchsin. It leaves it a nice pinkish tint after processing, the specimens
are visible in the paraffin, and we haven't had any pathologist complaints.
On Fri,
As much as I respect Dr. Richmond, I would have to disagree that staining
bx's with eosin is a waste of pathologist time. It helps the embedding
tech and cutting tech see the minute pieces, which may be otherwise lost.
Sometimes that is the diagnostic material.
We would not want to put a patien
I never noticed if ours shakes to rid the excess, but I do know the 95% alcohol
after eosin is pink but even when we stained manually, we had that as well.
What we do with the XL is rotate the 2nd 95% to that position and put fresh 95%
in the 2nd position. However, we only do that daily, not after
I think that you should talk with the person who sold you this refurbished
instrument and present your problem. That will be more expeditious.
René J.
--- On Fri, 1/15/10, Cheri Miller wrote:
From: Cheri Miller
Subject: [Histonet] re; eosin
To: "histonet"
Date: Friday, January 15, 2010, 1:04
Cheri Miller
To: "Sherwood, Margaret" ; "Breeden, Sara"
; "Mahoney, Janice A" ;
histonet ;
"histonet-boun...@lists.utsouthwestern.edu"
Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.
We do this as well, that is why I am
,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also tr
boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
Eosin is alcohol-based,
Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that
...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
[lbla...@digestivespecialists.com]
Sent: Wednesday, July 01, 2009 1:47 PM
To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Eosin in alcohol
We use about the same amount or eosin in the first 100% alcohol and also do
The eosin does not affect antibody binding but might interfere with
interpretation if they are doing multi-immunostaining on prostates and use a
red chromogen. Then the eosin may give an appearance of background. Or maybe
they just don't like the color introduced.
Tim Morken
Supervisor, Histolo
I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.
Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.
Jennifer, is it possible that the lab asking you to use cobalt blue is using a
fluorescence technique on the prostates? We've used eosin for years and not
seen any impact on IHC staining for light microscopy.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of Sou
We use about the same amount or eosin in the first 100% alcohol and also do
immunos on the biopsies and have no problem. We do all GI biopsies though not
prostate. I'm not sure why the eosin would interfere with the immuno staining
since it disappears during the hydration and retrieval process
We use eosin on the gross bench. We keep a bottle of concentrated Eosin
and Hematoxylin on the bench with an eyedropper. The eosin is for
endoscopic biopsies and the hematoxylin is for prostate biopsies. Neither
has never interfered with IHC staining.
Debbie M. Boyd, HT(ASCP),Chief Histologi
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