We try and find a mouse anti-rat antibody if we can and run rat secondary
(Dako makes one)
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-Original Message-
From: histonet-b
Just make sure you use an anti-mouse IgG antibody that is not cross
reactive to rat IgG. Jackson ImmunoResearch is the best place to find
these reagents ... I suggest the donkey as host, it works best for
me, least background. Beautiful results ...
Good afternoon.
I just had someone ask me ab
Hi:
We routinely perform immunohistochemistry (IHC) to detect antigens on
rat tissues. A mouse mAb should be fine for use in your rat samples.
You will not need a special kit, just an anti-mouse secondary Ab. One
runs into trouble when they use a mouse (or rat) primary on mouse (or
rat) tissue,
