Re: [Histonet] Xylene substitutes

Pros: aliphatic hydrocarbons are almost odorless and much safer than xylene Cons: they take a little longer to clear than xylene and they are not compatible with all mounting media -Original Message- From: Jacque Sagasser via Histonet Sent: Thursday, July 18, 2024 6:38 AM To: Histonet

Re: [Histonet] Xylene recycle or purchase fresh

You may wish to consider consulting with your local fire officer to find out what requirements are for installing a recycling unit in your facility. You may not even be allowed to place one. Also, consider the fumes from recycling xylene and if you want your expensive lab labour to be taken off

Re: [Histonet] Xylene substitutes for clearing agents

Under separate cover I am sending you 3 papers of mine that answer your question.René On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet wrote: Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on

Re: [Histonet] Xylene substitutes for clearing agents

Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire

Re: [Histonet] Xylene free processing

Cindy McGrady at Hurley Medical Center in Flint, Michigan asks: >>Our laboratory is experimenting with xylene free processing on our Sakura VIP, substituting isopropanol for our xylene substitute. Our pathologists have complained about processing, especially the biopsies, with our regular processi

Re: [Histonet] Xylene resistant gloves and xylene replacers (Piche, Jessica)

Good Morning, We are currently using XS-3 from StatLab and Formula 83 from CBG Biotech. Both of these are xylene substitutes and both are aliphatic hydrocarbons. They are less toxic than xylene, and don't have the strong smell we all learn to love about xylene. Limonene based substitutes are also

Re: [Histonet] Xylene Free

I am also interested in that i run a small lab and would love to go xylene free! 2017-02-23 12:21 GMT-06:00 Blake Taylor via Histonet < histonet@lists.utsouthwestern.edu>: > Our hospital has asked us to investigate going xylene free. Has anyone > recently made a switch to a xylene alternative an

Re: [Histonet] Xylene and Formalin substitutes

Hey, The previous supervisor switched the Histo lab to CBG's Formula 83 with no problems as our xylene substitute. In fact, we even distil it and use the distilled in both our staining and processor. The only con is that it is not as effective in removing coverslips from slides. Unfortunate

Re: [Histonet] Xylene and Formalin substitutes

The most straight forward solution to your problem is the following: 1- change from ethanol dehydration to 2-propanol (isopropyl-alcohol)2- after 100% propanol, go directly a mixture 1:1 of propanol with mineral oil light molecular weight3- go to your regular paraffin embedding steps. These chang

Re: [Histonet] Xylene and Formalin substitutes

Good Morning! Our lab actually just went through some research regarding this, so I thought I would share. We have used the xylene substitute Americlear from Cardinal Health for a long time. It works well with our reagent alcohols and formalin, and we haven't had any processing or staining iss

Re: [Histonet] Xylene and Formalin substitutes

Contact Ada Feldman, Anatech Ltd. Best resource. Sent from my Windows Phone From: Bharti Parihar via Histonet Sent: ‎10/‎21/‎2015 3:49 PM To: histonet@lists.utsouthwestern.edu Subje

Re: [Histonet] Xylene Free Labs - One Final Question

--Original Message- From: "Rene J Buesa" To: "Brendal Finlay" , histonet@lists.utsouthwestern.edu Date: 08/13/2015 15:33 Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions   1-After you oven dry your stained sections, you use the very sam

Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminate

Re: [Histonet] Xylene substitutes in staining line

You wrote: Hello Histo Land, Does anyone use xylene substitutes in their stain line only? I am especially interested in d-limonene options. Thanks in advance! * We used

Re: [Histonet] Xylene Substitutes

We used NOTHING in our staining line. We dewaxed with 2% dishwasher soap followed by the normal staining (no xylene → no ethanol).After staining we dried the stained section in an oven at 60ºC for 15 minutes → coverslip (no ethanol → no xylene).To process we used iso-propanol → mineral oil → par

Re: [Histonet] Xylene Substitute Protocols

We have found that Permount works best with the aliphatic hydrocarbons. Sent from my iPad > On Jul 24, 2014, at 10:01 AM, "Hilliard, Dawud Deshawn" wrote: > > Hello all, > > Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type fo

Re: [Histonet] Xylene substitute for processing GI biopsies

s.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can

RE: [Histonet] Xylene substitute for processing GI biopsies

nt: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher s

Re: : [Histonet] Xylene substitute for processing GI biopsies

Hi Lori, Pretty much any xylene sub will work for processing tissues and staining. You may need to add additional time in the "xylene" steps, since the action of the subs isn't as quick as xylene. In addition, the substitutes are more intolerant of water. How humid is your lab? If it's dry, the

RE: [Histonet] Xylene substitute for processing GI biopsies

: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, April 18, 2014 10:41 AM To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsie

RE: [Histonet] Xylene substitute for processing GI biopsies

tonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with

RE: [Histonet] Xylene substitute for processing GI biopsies

n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known

Re: [Histonet] Xylene substitute for processing GI biopsies

Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coversli

Re: [Histonet] Xylene Resistant Labels

Hi Schaundra, I have used the StainerBondz labels from Brady for quite a while, and am quite pleased with them. Good luck! Sean McBride Senior Scientific Specialist Bone Tissue Engineering Center Research Histology Services Carnegie Mellon Research Institute Suite 4311 700 Technology Drive P

RE: [Histonet] Xylene Resistant Labels

We use a printer that we purchased from General Data and their labels and they created a specialized software for us since we are a research lab. The labels work great in our hands. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3

Re: [Histonet] Xylene Substitutes

u'" Sent: Wednesday, August 21, 2013 12:20 PM Subject: RE: [Histonet] Xylene Substitutes Rene, How do you recommend cover slipping with out xylene?  We use a tape cover slip instrument that requires xylene.  If you are using glass coverslips what kind of adhesive is used?  I am

RE: [Histonet] Xylene Substitutes

= tachment, is prohibited. Original M= essage Subject: Re: [Histonet] Xylene Substitutes From= : Rene J Buesa <[2]rjbu...@yahoo.com> Date: Wed, August 21, 2013 12:06 pm To:"Yaskovich,Ruth=A(NIH/NIDCR)[E]"

Re: [Histonet] Xylene Substitutes

Ruth: I all reality none is good. Both alkanes or D-Limonene are either as dangerous or more than xylene, and all perform below the xylene standard. Any one requires modifications of all procedures, they seldom dewax adequately and all constitute a processing "compromise". Many cannot de recycled

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitutes for tissue processor clean cycle I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck. V.Ava

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

We use Americlear. Have not used Xylene for many, many years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:his

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

Carla, We use Formula 83 in the clean cycle. We do change it more often than you would xylene though. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 11

RE: [Histonet] Xylene substitutes for tissue processor clean cycle

Carla I think most tissue processor vendors recommend xylene for cleaning and do not recommend a xylene substitute. This may have changed recently but I would suspect it would void your warranty if you were still under one if the vendor recommends xylene and you are using a xylene substitute f

Re: [Histonet] Xylene substitutes for tissue processor clean cycle

Use a 5% solution of a strong lab detergent in isopropyl alcohol. Since the cleaning cycle is completed at 50ºC that will be enough and you will not have to expose yourself to xylene vapors. René J. From: "Conway, Carla" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 21, 2013 11:25 A

Re: [Histonet] Xylene/Paint Thinner

I have to admit, I don't mind the smell:) But I also like the smell of gasoline, and I know both are bad for me! Thanks again to everyone who has offered feedback on this topic. Rene, I have printed out a few of the articles you've written and am anxious to research these methods more. Thanks a

Re: [Histonet] Xylene/Paint Thinner

For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the "like the smell of xylene". René J. From: "White, Lisa M." To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:23 AM Subject: [Histonet] Xylene/Paint Thinner If you look

Re: Re: [Histonet] Xylene Substitutes

n'; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 5:36 AM Subject: RE: [Histonet] Xylene Substitutes Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a lit

Re: [Histonet] Xylene/Paint Thinner

You can find the actual chemical composition if you Google the name and look for the MSDS (as Dr. Richmond has suggested several times). René J. From: "Parker, Helayne" To: "'histonet@lists.utsouthwestern.edu'" Sent: Wednesday, February 6, 2013 1:27 PM Subject: [Histonet] Xylene/Paint Thinner

RE: [Histonet] Xylene Substitutes

Dessoye, Michael J Sent: Wednesday, February 06, 2013 11:47 AM To: Adrienne Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Substitutes We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye

RE: [Histonet] Xylene Substitutes

We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes

Re: [Histonet] Xylene Substitutes

ot; Sent: Wednesday, February 6, 2013 8:30 AM Subject: Re: [Histonet] Xylene Substitutes Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013,

Re: [Histonet] Xylene Substitutes

strong lab lab-ware detergent. Do all of the above and your lab will be xylene free. René J. From: Tom McNemar To: 'Adrienne Anderson' ; "histonet@lists.utsouthwestern.edu" Sent: Wednesday, February 6, 2013 5:36 AM Subject: RE: [Histonet] Xylene Substitutes Not familiar w

RE: [Histonet] Xylene Substitutes

...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Wednesday, February 06, 2013 6:30 AM To: Tom McNemar Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Substitutes Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the

Re: [Histonet] Xylene Substitutes

Thanks so much, everyone, for the feedback. I have a colleague who is convinced paint thinner is exactly the same as the SubX we use. Does anyone know if this is true? Thanks again, Adrienne On Feb 6, 2013, at 5:36 AM, Tom McNemar wrote: > Not familiar with SubX but we have used Americlear f

RE: [Histonet] Xylene Substitutes

We use Pro Par Clearant through Fisher from Anatech. Health-1, Flammablity-2, Instability-0. For a case of 4 gallons it runs us about $170. Janine Simms Colon, CPhT, HT(ASCP), BHA Histology/Pathology Johnson Memorial Hospital 201 Chestnut Hill Road Stafford Springs, CT 06076 Office: 860-684-8230 e

RE: [Histonet] Xylene Substitutes

Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org

Re: [Histonet] Xylene

Unfortunately you are right about xylene being preferentially used although at present the number of labs using it has been reduced to about 60%. You are also right that, compared with other "traditional" products, xylene produce results that can be considered as the "gold standard", but it is hi

Re: [Histonet] Xylene free processing

Not even to clean the tissue processor you will need xylene if you prepare a 5% solution of liquid dish washer soap in isopropyl alcohol. Place this solution in the cleaning "used xylene" container and clean the tissue processor with it. René J. From: "Martin, Erin" To: histonet Sent: Thursda

RE: [Histonet] Xylene Free Processing?

Bill Sent: January 9, 2013 12:16 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Free Processing? The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcoho

RE: [Histonet] Xylene Free Processing?

western.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 09, 2013 2:46 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Free Processing? Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. René J. From: &qu

Re: [Histonet] Xylene Free Processing?

/rene.html René J.  From: "O'Donnell, Bill" To: "Jones, Laura" ; Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 3:15 PM Subject: RE: [Histonet] Xylene Free Processing? The Xpress microwave tx processors are xylene free - perhaps they have one. I am

Re: [Histonet] Xylene Free Processing?

Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. René J. From: "Jones, Laura" To: "Histonet@lists.utsouthwestern.edu" Sent: Wednesday, January 9, 2013 2:37 PM Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expe

RE: [Histonet] Xylene Free Processing?

The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most p

RE: [Histonet] Xylene Substitute for Counterstain Clearing

. Good luck! Gayle Callis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 11, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu; Andrew Coleman Subject: Re: [Histonet

Re: [Histonet] Xylene Substitute for Counterstain Clearing

After staining dry the sections in an oven at 60ºC for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. René J. --- On Fri, 5/11/12, Andrew Coleman wrote: From: Andrew Co

Re: [Histonet] Xylene Substitute for Counterstain Clearing

I think that it is called Crystal Mount - but apply to section, allow to harden dip slide in clearing media and coverslip. I know that there must be others out there as well. Good Luck On Fri, May 11, 2012 at 12:25 PM, Andrew Coleman wrote: > Hi all, > > We are performing a neutral red counte

RE: [Histonet] Xylene Substitute for Counterstain Clearing

Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Origina

RE: [Histonet] Xylene Substitute for Counterstain Clearing

Andrew There are aqueous permanent mounting medias such as Advantage Permanent Mounting Media from Accurate Chemical NB300A (516) 333-2221 its been years since I used it but it does work on some applications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 1

Re: [Histonet] xylene substitute for processing

Kelly, We uses for years mineral oil instead xylene for overnight manual processing and have seen not any adverse effect in H&E, HC and IHC. Opposite, we noticed, that our slides becomed better, crisp and clear than with xylene. Mineral oil have also many positive additional moments for techs and l

Re: [Histonet] Xylene and Preggers

Human resources ultimately represents the company . A pregnant employee should be wearing ppe when handling xylene. MSDS forms are accessible to all employees, just a click away on the computer. I can understand wanting to keep some things private until you are ready to communicate them, but wh

RE: [Histonet] Xylene alternative

Toni, Slide bright is everywhere xylene would have been. Proceesor, stainline, etc. I use either TBS shur/mount( xylene based) or S mounting media (toulene based). I think the xylene based media works better. As per the directions it can be flushed down the drain with copious amounts of water. B

RE: [Histonet] Xylene alternative

Amber, I have a dermpath/ mohs lab and I use slide bright for everything. I have used the product for 9yrs now, at least. Slide bright is in my processor, H%E stain line and my deparrafin stain line. Definitly call and get sample. I love it. I get mine from Mercedes medical, belair(advantik), PSS

RE: [Histonet] Xylene alternative

Do you use this product for processing and staining? Do you need to use a particular type of mounting medium? How is it disposed of? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Wedne

Re: [Histonet] Xylene

Might not be toxic but the fumes of citrus give me headaches and the smell is terrible.. Im a slide bright girl, no odor, and non flammable Hello Histonetters > I have been reading about xylene, how dangerous it is. > Have you thought about using mounting medium made from Limonene. > Here is some

RE: [Histonet] Xylene sensitivity

To: D'Attilio, Shelley Cc: Histonet Listserv (E-mail); histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene sensitivity I understand the reluctance to discontinue the use of the xylene in the tissue processor for FDA compliance, but why not change the solvent for stainin

RE: [Histonet] Xylene sensitivity

.edu 09/27/2011 01:25 PM To "Sarah Holmes" cc "Histonet Listserv \(E-mail\)" Subject RE: [Histonet] Xylene sensitivity Hi Sarah, Thanks so much for the information. We do have a small fume extractor next to our formalin containers in the Gross Room. This might be worth

RE: [Histonet] Xylene sensitivity

Rene', Thanks for the quick response to my question. The two articles that you sent were very interesting and I have forwarded them to my department histotech supervisor for her evaluation. It is apparent from your comments on the listserv that you have a strong commitment to safety. You h

RE: [Histonet] Xylene sensitivity

Hi Jan, Thanks for the advice on xylene substitutes. Your symptoms mirror those of my affected employee and I'm glad to hear that you found relief using another product. I'm anxious to fix this situation as quickly as possible. This employee is ready to make a career of Histology and I would

RE: [Histonet] Xylene sensitivity

, Loralee A; Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio Subject: RE: [Histonet] Xylene sensitivity Can ya'll take this argument off-line? Obviously xylene is staying in Loralee's lab and Rene thinks Loralee is killing her employees. We all get it now. No real benefic

RE: [Histonet] Xylene sensitivity

larger version of that type of device, so it might work quite well. Thanks for taking the time to respond, Shelley -Original Message- From: Sarah Holmes [mailto:sa...@kidneybiopsy.com] Sent: Tuesday, September 27, 2011 12:22 PM To: D'Attilio, Shelley Subject: Re: [Histonet] X

RE: [Histonet] Xylene sensitivity

western.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, September 27, 2011 2:59 PM To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio Subject: RE: [Histonet] Xylene sensitivity We did not assume anything. My sister hospital does not perform any immunos, we do all the immunos

RE: [Histonet] Xylene sensitivity

ttilio; McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity You are assuming that the decline in your "sister" lab was due to not using xylene and you should never assume. I would have taken several cases of mine, divided in two parts and given your "sister" lab hal

RE: [Histonet] Xylene sensitivity

René J.   --- On Tue, 9/27/11, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" Date: Tuesday, September 27, 2011, 1:09 PM Well actual

RE: [Histonet] Xylene sensitivity

ng Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, September 27, 2011 11:35 AM To: Histonet Listserv (E-mail); ShelleyD'Attilio; McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity

RE: [Histonet] Xylene sensitivity

@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene sensitivity Rene', I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everything has been standardized/validated another employee begins employment, but exhibits sensitivity to the n

RE: [Histonet] Xylene sensitivity

@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene sensitivity Glen, I suppose it would all depend upon which xylene substitute one uses - if one switches to another that has a pungent odor such as the citrus based solvents - there could be problems with that as well.

RE: [Histonet] Xylene sensitivity

of many! Kelly -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Tuesday, September 27, 2011 11:27 AM To: rjbu...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xyl

RE: [Histonet] Xylene sensitivity

om: Glen Dawson Subject: RE: [Histonet] Xylene sensitivity To: rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu Date: Tuesday, September 27, 2011, 12:26 PM Rene',   I must respectfully ask: what happens after you modify your procedures for this new employee & 2 days after everythi

RE: [Histonet] Xylene sensitivity

n.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 27, 2011 7:55 AM To: Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon Subject: RE: [Histonet] Xylene sensitivity Loralee: Please enlighten me! As far as I know FDA requirements

RE: [Histonet] Xylene sensitivity

Date: Tue, 27 Sep 2011 08:44:35 -0700 From: rjbu...@yahoo.com Subject: RE: [Histonet] Xylene sensitivity To: sdatt...@stormontvail.org; histonet@lists.utsouthwestern.edu; ihcman2...@hotmail.com Glen: I respectfully disagree. Do you have any idea what would mean to that employee to t

RE: [Histonet] Xylene sensitivity

tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Cross Sent: Tuesday, September 27, 2011 11:49 To: Rene J Buesa; Histonet Listserv (E-mail); ShelleyD'Attilio; Loralee AMcMahon Subject: RE: [Histonet] Xylene sensitivity I have gone ar

RE: [Histonet] Xylene sensitivity

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Tuesday, September 27, 2011 10:11 AM To: sdatt...@stormontvail.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene sensitivity Shelley, Perhaps this employee should look into other job opportunities. The

RE: [Histonet] Xylene sensitivity

-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 27, 2011 10:45 AM To: sdatt...@stormontvail.org; histonet@lists.utsouthwestern.edu; Glen Dawson Subject: RE: [Histonet] Xylene sensitivity Glen: I respectfully dis

RE: [Histonet] Xylene sensitivity

); ShelleyD'Attilio; Loralee AMcMahon Subject: RE: [Histonet] Xylene sensitivity Loralee: Poor people in your sister hospital! You are affecting their health. I would have approached a different way and never done that. DAKO cannot dictate how I process my samples. I would have gotten involved my

RE: [Histonet] Xylene sensitivity

matter how much we are "used to". René J. --- On Tue, 9/27/11, Glen Dawson wrote: From: Glen Dawson Subject: RE: [Histonet] Xylene sensitivity To: sdatt...@stormontvail.org, histonet@lists.utsouthwestern.edu Date: Tuesday, September 27, 2011, 11:11 AM Shelley, Perhaps this emplo

RE: [Histonet] Xylene sensitivity

1, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio" Date: Tuesday, September 27, 2011, 10:57 AM The Hercept Test from Dako specifies  xy

RE: [Histonet] Xylene sensitivity

Shelley, Perhaps this employee should look into other job opportunities. The histo lab is filled with chemicals that can cause sensitivity in those people who may be prone to that. Unfortunately, some of the main players in the histo lab (xylene & formalin) are often the culprit, but they a

RE: [Histonet] Xylene sensitivity

pitopes. I know that you cannot argue with FDA, but you can always validate any changes in your procedure. René J. --- On Tue, 9/27/11, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity To: "Rene J Buesa" , "Histonet Listserv (E-mail)"

RE: [Histonet] Xylene sensitivity

with FDA, but you can always validate any changes in your procedure. René J. --- On Tue, 9/27/11, McMahon, Loralee A wrote: From: McMahon, Loralee A Subject: RE: [Histonet] Xylene sensitivity To: "Rene J Buesa" , "Histonet Listserv (E-mail)" , "ShelleyD'Attilio&

RE: [Histonet] Xylene sensitivity

tilio Subject: Re: [Histonet] Xylene sensitivity Shelley: As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution. Is like having a pain when you use your arm and immobilizing it to prevent the pain. Your long term and complete soluti

Re: [Histonet] Xylene sensitivity

Shelley: As you correctly think this employee is most likely sensitive to xylene. Having a filtration system is the wrong solution. Is like having a pain when you use your arm and immobilizing it to prevent the pain. Your long term and complete solution is to eliminate xylene from your lab and I

Re: [Histonet] xylene

glass coverslips On Thu, Nov 11, 2010 at 12:50 PM, Ranostaj, Drew wrote: > Are you coverslips tape or glass that you are trying to remove? > > Drew Ranostaj > Medite Inc. > 1226 Winter Garden Vineland Rd, Suite 104 > Winter Garden, Florida 34787, U.S.A. > > Phone: +1 (407) 996 9630 > Fax: +1 (407

RE: [Histonet] xylene

We use Formula 83 and we still have to use Xyene to remove coverslips from slides that are more than 2 days old. Joyce Cline Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 joyce.cl...@wchsys.org From: histonet-boun...@lists.utsouthwestern.edu

Re: [Histonet] xylene

My responses to some questions I have received (also FYI for anyone else with the same issue): Are you using the tape coverslips or the glass ones? If using the tape, it will only be removed with acetone. See what your mounting medium is based with (Xylene? Toluene? etc.,) and see if Formu

RE: [Histonet] Xylene Substitute

We use Clear-Rite 3 (Richard Allan Scientific 6901) from Fisher Scientific in our VIP processor and for staining. I am not familiar with Permount, but we use Richard-Allan Mounting Medium (4111; also available from Fisher Scientific) that is toluene based - works great in combination with Clear

RE: [Histonet] Xylene Substitute

Sheila, You might want to talk to the Leica rep. My guy was here a couple of weeks ago and they have a xylene sub and a mouting media that plays nicely with it. I don't recall the name of the product, but it might be worth talking to him about it. Good Luck! Michelle -Original Message

RE: [Histonet] Xylene/Solvent Waste

AM To: 'histonet@lists.utsouthwestern.edu'; Cortright, Valerie Subject: Re: [Histonet] Xylene/Solvent Waste That will depend on the original usage determined by the work volume. You will have to make your determination yourself for your particular conditions and the data from others will

Re: [Histonet] Xylene/Solvent Waste

That will depend on the original usage determined by the work volume. You will have to make your determination yourself for your particular conditions and the data from others will be only useful for comparative purposes, i.e. are you generating more or less waste than others. René J. --- On Th

Re: [Histonet] xylene in an unventilated area

I just recently did a lot of research into the different hoods. I decided the down draft was not going to work for us since we like to place absorbant towels down to catch the xylene while coverslipping. I decided to purchase one that a high CFM (Air flow rate), low decibles and a monitor o

Re: [Histonet] xylene in an unventilated area

Try to get a fume hood; it could be one movible over the counter, but needs to have a good charcoal filter. René J. --- On Mon, 8/16/10, cathy.crump...@tuality.org wrote: From: cathy.crump...@tuality.org Subject: [Histonet] xylene in an unventilated area To: histonet@lists.utsouthwestern.edu

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