To store cut sections for use later for IHC I do not bake them to melt the paraffin, they keep better, melt them just before use. I also store the cut slides at 4dc. For really precious control blocks, I reseal them with paraffin (just dip in hot paraffin and then cool) and I do also store the blocks as 4dc. We just ran into a problem with tonsil sections going bad for IMP3 ab., this is a real concern.
Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Saby Sent: Friday, May 14, 2010 4:21 PM To: Perry, Samuel; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Prolonged FFPE slide and block storage Sam- Are you storing sectioned slides? What we do is to deparaffinize and coverslip sectioned but unstained slides. I haven' had to go back, but I believe they will store that way a long time. Joe Saby, BA HT ________________________________ From: "Perry, Samuel" <samuel_pe...@dfci.harvard.edu> To: histonet@lists.utsouthwestern.edu Sent: Fri, May 14, 2010 4:16:06 PM Subject: [Histonet] Prolonged FFPE slide and block storage Hi All, We have a growing need for storing slides. They need to be stored in a way that they won't become oxidized and loose their antigenicity, since we use them for IHC. Any suggestions for inexpensive methods for prolonged storage of FFPE slides and blocks is greatly appreciated. Thanks! Sam Perry Research Technician Dana-Farber Cancer Institute Boston, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet