information.
dusko
From: "Zerfas, Patricia (NIH/OD/ORS) [E]"
To: dusko trajkovic
Sent: Monday, August 10, 2009 12:07:29 PM
Subject: RE: [Histonet] CD31 working protocol
Dusko,
I am using mouse tissue.
Patricia Zerfas
National Institutes of Healt
Hi Patricia,
What tissue (animal / human) are you using? How has it been fixed / processed?
If you are using 10%NBF fixed, paraffin-embedded mouse tissue, you can
use Biocare's CD31 with a simple indirect protocol. I come in with my primary
(overnight at 5ug/ml) after Prot. K digestion (DA
Biocare has a CD31 anti mouse that does stain FFPE.I personally think
nothing will ever compare with the Santa Cruz goat that no longer is
available - but Biocare does work.
"Zerfas, Patricia (NIH/OD/ORS) [E]"
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/10/2009 01:50 PM
To
"
Biocare has a great CD31 for mouse and rat too. You do need the kit and
their primary but you can use formalin fixed samples with great results!
Colleen Forster
U of MN
Amy Porter wrote:
BD Biosciences has a great rodent CD31 for mouse and rat - however you
need to use their Zinc Fixative. I
BD Biosciences has a great rodent CD31 for mouse and rat - however you need
to use their Zinc Fixative. It is well worth the results!!
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
