Re: [Histonet] I have a couple of quick questions

2010-10-15 Thread Rene J Buesa
Yes, xylene is the way to go. No, 37ºC will not hurt the sections no matter for how long they are at that temp. René J. --- On Thu, 10/14/10, Lewis, Patrick wrote: From: Lewis, Patrick Subject: [Histonet] I have a couple of quick questions To: Histonet@lists.utsouthwestern.edu Date: Thursday,

Re: [Histonet] I have a couple of quick questions

2010-10-15 Thread BSullivan
Yes. to remove mounting media you need to leave them in the Xylene after the coverslip is removed. If the mounting media is not totally removed your stain, whatever one you are doing, will not give you the results you need. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor

Re: [Histonet] I have a couple of quick questions

2010-10-14 Thread godsgal...@aol.com
Yes, soak slides in xylene to remove coverslip. A few days in 37 degree oven won't hurt Roxanne Sent from my Verizon Wireless Phone - Reply message - From: "Lewis, Patrick" Date: Thu, Oct 14, 2010 7:11 pm Subject: [Histonet] I have a couple of quick questions To: If I want to remov