Laurie,
The warm water helps the color of the Schiff develop in a more vibrant pattern.
If you allow the slides to sit in warm water for 2 min, then rinse you also do
not have to use the metabisulfite rinses to remove excess stain.
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Assistant Professor/Edu
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Subject: Re: [Histonet] PAS Stain
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a f
As I see it, there are 3 main objections about using human saliva as an amylase
source.In order of importance they are:1- you will never know the actual
concentration of the amylase and this will produce reproducibility problems.2-
along with the saliva you will introduce bacteria that may end b
n via Histonet"
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 5, 2016 5:30:22 PM
Subject: Re: [Histonet] PAS Stain
Hello All! I've served in laboratory medicine for well over 35 years and in my
early years we did indeed spit on the slides. I learned very quickly on that my
e even
involved his family! Dedicated doctors...
Claire
From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 6:33 PM
To: Anne Murvosh
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
rvosh
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
Only a crazy Aussie would do this!!
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, Uni
: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
You clearly don't know your histo history. The reason we know that H pylori
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria
caused ulcers and not stress. No one believed him. So he took the organisms
from a
estern.edu
Subject: Re: [Histonet] PAS Stain
I cannot believe any scientist would advocate such a non-uniform method
as spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and
temperature.
Geoff
On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
> Yes, spitting
slide... :)
Claire
From: Ray via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 1:48 PM
To: Richmond, Bob
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
An excellent point. For anyone wanting to investigate-simp
k the best. A fun experiment Anne
-Original Message-
From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 11:36 AM
To: koelli...@comcast.net
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain
Spokane Ray point
n.edu
Subject: Re: [Histonet] PAS Stain
Spokane Ray points out something I've wondered about for years - can just
anybody spit on the slide and remove the glycogen? I've never heard of any
variation, but the number of people I've asked is very limited. This
reference:
http://www.ncbi.
Cc: "Histonet@lists.utsouthwestern.edu"
Sent: Thursday, May 5, 2016 11:35:42 AM
Subject: Re: [Histonet] PAS Stain
Spokane Ray points out something I've wondered about for years - can just
anybody spit on the slide and remove the glycogen? I've never heard of any
variation, but the
Hello
Agree with comments about temperature of 60 degrees killing an enzyme. If you
plot enzyme activity on "y" axis and temperature on "x" axis it is not a
straight line. Every enzyme has an optimal temperature and can function slowly
at non-optimal or optimally at correct temperature.
We switched form malt diastase to alpha amylase and have seen a
significant improvement in digestion, but as others have said enzyme
activity will be destroyed at 60 degrees C. We put ours into a 37 degree C
water bath. Enzymes work optimally at 37C, body temperature. As the
temperature rises
Why not just standardize it from the start, reagent, pH, temperature and
> it really cannot fail.
>
> Spokane Ray
>
> --
> *From: *"Bob Richmond via Histonet"
> *To: *"Histonet@lists.utsouthwestern.edu" <
> histonet@lists.u
Histonet"
To: "Histonet@lists.utsouthwestern.edu"
Sent: Thursday, May 5, 2016 11:10:40 AM
Subject: Re: [Histonet] PAS Stain
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinki
Amylase (diastase) for the PAS stain queries:
Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.
He notes that alpha amylase is preferred.
We switched to alpha amylase since the diastase we were ordering was not
working. We use a 2% solution of alpha amylase for 40 minutes at room
temperature and it has been working fine.
Phil.
Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Bo
As far as I remember the incubation temperature is at roomtemperature. 60°C
would rather denature the native enzyme than increase activity.
Look at the optimal working temp of the reagens you have bought.
regards
Gudrun
-Ursprüngliche Nachricht-
Von: Joanne Clark via Histonet [mailto:his
We use the hematoxylin that is used on the H&E stainer. While I know it is not
optimal, the pathologists should be used to looking at it so it makes things a
lot easier. The time is cut down to less than 30 seconds and we do not use a
differentiator.
For the students, it helps them to underst
Hi Mica,
Most haematoxylins will do eg Harris or Mayer's.
I would recommend a light counterstain (it is a counterstain, not the main
stain).
Differentiate if too strong.
Also periodic acid treatment increases nuclear affinity for Hx so shorten the
counterstain time.
Regards,
Tony
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8201 East Riverside Dr. Bldg 4 Suite 100
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(512)386-5107
Original Message
Subject: Re: [Histonet] PAS STAIN
From: Geoff McAuliffe <[1]mcaul...@um= dnj.edu>
Date: Wed, October 06, 2010 1:28 pm
To: Diana McCaig <[2]d
I make my Schiff's from scratch and store it in the refrigerator.
I use a minimum amount of charcoal and I heat it in the oven at over 100
C the night before to be sure it is nice and dry.
I make periodic acid fresh that day.
I make bisulfite rinses fresh that day.
Aqueous formalin for 48 hours
tonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, October 06, 2010 9:06 AM
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] PAS STAIN
Were the new bottles from the same lot?
-Original Message-
From
Were the new bottles from the same lot?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana
McCaig
Sent: Wednesday, October 06, 2010 11:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS S
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