RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-14 Thread Tony Henwood
Christina, The following might be useful: Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from formalin fixed lung tissue that had previously been shown to be positive by sputum culture. They were unable to culture TB from these tissues. Gerston et al (HUM PATHOL 35:571-575.

RE: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Kumar, Devender
sage- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 13, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu; ChristinaThurby Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in

Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Rene J Buesa
5 days (96 h) is more than enough, but always handle the tissue and blocks using safety precautions. René J. --- On Wed, 4/13/11, Thurby, Christina wrote: From: Thurby, Christina Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case? To: "hist

Re: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation for a suspected TB case?

2011-04-13 Thread Jay Lundgren
As long as possible. I remember reading somewhere about TB in lung tissue that was still viable after *years* of 10% NBF. Make sure the lungs are thoroughly perfused, or they will still be unfixed in the middle. There is a technique to "inflate" lungs at autopsy using a large syringe full o