Christina,
The following might be useful:
Kappel et al (HUM PATHOL 27:1361--1364, 1996) attempted to grow TB from
formalin fixed lung tissue that had previously been shown to be positive by
sputum culture. They were unable to culture TB from these tissues.
Gerston et al (HUM PATHOL 35:571-575.
sage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, April 13, 2011 3:57 PM
To: histonet@lists.utsouthwestern.edu; ChristinaThurby
Subject: Re: [Histonet] RE: How long should autopsy/necropsy tissue be in
5 days (96 h) is more than enough, but always handle the tissue and
blocks using safety precautions.
René J.
--- On Wed, 4/13/11, Thurby, Christina wrote:
From: Thurby, Christina
Subject: [Histonet] RE: How long should autopsy/necropsy tissue be in fixation
for a suspected TB case?
To: "hist
As long as possible. I remember reading somewhere about TB in lung
tissue that was still viable after *years* of 10% NBF. Make sure the lungs
are thoroughly perfused, or they will still be unfixed in the middle. There
is a technique to "inflate" lungs at autopsy using a large syringe full o