Hi David,

Thank you for your quick and honest response. I am sorry to hear that you
have no funds to continue TPP implementation. I hope this problem will be
solved.
Nevertheless, I appreciate your consideration of evaluating a sample file.
This file contains what the PEAKS team calls ‘Peptide output in pepxml’. I
hope it is adequate but in any case I don't think there will be a problem
in getting a suitable data output for TPP if necessary. The other format
that can be obtained is mzidentML in case it is of interest.

LINK: pepXML PEAKS sample
<https://drive.google.com/file/d/185t5m7vJPLQAj8vVpcviX_p58DHZfyAB/view?usp=sharing>

Thanks again for your reply.

Best regards,
Sergio

--------------------------
Sergio Ciordia Higuera
Proteomics Facility
National Center for Biotechnology
C\Darwin, 3
Universidad Autónoma de Madrid
Cantoblanco
28049 Madrid (Spain)
Phone: +34 91 585 4540 / 4695
Fax: +34 91 585 4506


El jue, 10 oct 2024 a las 0:46, David Shteynberg (<
[email protected]>) escribió:

> Hello Sergio,
>
> Thank you for your email.  As you know PEAKS is not a search engine that
> we have integrated in the TPP, mainly because we have not had any requests
> for this feature before your email.  It is something that can be done with
> a bit of work and testing, but unfortunately there is currently no funding
> for us to continue this work.  I wish I had a more satisfying answer to
> give you, but perhaps, if you can forward your sample pep.xml file, we can
> do this if more funding becomes available.
>
> Best,
> -David
>
> On Oct 9, 2024, at 3:35 PM, Sergio Ciordia <[email protected]> wrote:
>
> Hi,
>
> I have been using TPP for some time now mainly to validate with
> PeptideProphet the results I get with various search engines and to
> generate a spectral library that I use in various programs. The thing is
> that in my lab we are now using PEAKS (v12) and the output is really good.
> I was wondering if it would be possible to include in TPP an analysis
> pipeline of the PEAKS data from the pep.xml file generated by the program.
> I know it is a commercial software but the output is very good and I would
> be very grateful if you could consider including it like Sequest or Mascot.
> I think it would not be complicated since they already have the pep.xml
> output, it would only have to be compatible with XInteract to be able to
> validate peptide-spectrum matches.
>
> If necessary I can provide a sample pep.xml file.
>
> Thank you very much.
>
> Best regards,
> Sergio
>
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