Re: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

2024-04-03 Thread Filate Fiche
Thank you for letting us know.  

On Wednesday, April 3, 2024 at 04:45:01 PM GMT+3, Lena Wilson 
 wrote:  
 
 Hi, PlantGENE members,
Thanks for all the helpful replies and information! I wanted to let you know 
that I have found a source for R. rhizogenes at my university.
Sincerely,Lena
On Wed, Mar 27, 2024 at 3:16 PM Lena Wilson  wrote:

Hello, PlantGENE forum,
I was wondering if anyone can point me toward sources for Rhizobium rhizogenes 
(Agrobacterium rhizogenes) for plant transformation? 
My location is at Cornell University in New York, USA, so an American source 
may be easiest.
Thank you,
Lena Wilsonlcw64@cornell.eduPhD studentWeber LabCornell University
P.S. This seems to be the first PlantGENE forum message, so fingers crossed 
that this goes well!
  

Re: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

2024-04-03 Thread Lena Wilson
Hi, PlantGENE members,

Thanks for all the helpful replies and information! I wanted to let you
know that I have found a source for *R. rhizogenes* at my university*.*

Sincerely,
Lena

On Wed, Mar 27, 2024 at 3:16 PM Lena Wilson  wrote:

> Hello, PlantGENE forum,
>
> I was wondering if anyone can point me toward sources for Rhizobium
> rhizogenes (Agrobacterium rhizogenes) for plant transformation?
>
> My location is at Cornell University in New York, USA, so an American
> source may be easiest.
>
> Thank you,
>
> Lena Wilson
> lc...@cornell.edu
> PhD student
> Weber Lab
> Cornell University
>
> P.S. This seems to be the first PlantGENE forum message, so fingers
> crossed that this goes well!
>


RE: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

2024-03-27 Thread Thilmony, Roger - REE-ARS
Hi Lena,

Are you looking for a disarmed A. rhizogenes strain, or are you looking for a 
wildtype strain that will generate hairy roots (and is still pathogenic and can 
cause disease)?

I am not sure what permit restrictions Vladimir is referring to, but as far as 
I know, disarmed (nonpathogenic) Agrobacterium strains do not have APHIS 
restrictions in their use or shipment (--although a movement notification used 
to be required to ship a strain from one state to another within the US, that 
is no longer required for nonpathogenic disarmed strains).

Best,
Roger


Roger Thilmony Ph.D.
USDA-Agricultural Research Service
Western Regional Research Center
Crop Improvement and Genetics Research Unit
800 Buchanan Street
Albany, CA 94710-1105


From: bounce-128114351-97148...@list.cornell.edu 
 On Behalf Of Orbovic,Vladimir
Sent: Wednesday, March 27, 2024 12:23 PM
To: Lena Wilson ; plantgenememberforu...@cornell.edu
Subject: RE: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

Hello Lena,

The easiest way to get R rhizogenes would be from ATCC.org (in the state of 
Virginia). However, I hope you have a permit to use it. The APHIS has recently 
changed regulations in respect to this species and you have to receive permit 
to be able to use it. I am in the process of getting the permit now. Hope this 
helps.

Vladimir

Vladimir Orbovic PhD
Citrus Research and Education Center
University of Florida/IFAS
700 Experiment Station Road
Lake Alfred, FL 33850
Phone 863-956-8729
Fax 863-956-4631
For the podcast on The Use of Genetic Engineering in Fruit Breeding please 
click 
here<https://www.scipod.global/harnessing-genetics-for-sustainable-fruit-production-dr-vladimir-orbovic-university-of-florida/>
Google Scholar Profile click 
here<https://scholar.google.com/citations?user=3EkgV00J=en>


From: 
bounce-128114308-97181...@list.cornell.edu<mailto:bounce-128114308-97181...@list.cornell.edu>
 
mailto:bounce-128114308-97181...@list.cornell.edu>>
 On Behalf Of Lena Wilson
Sent: Wednesday, March 27, 2024 3:17 PM
To: 
plantgenememberforu...@cornell.edu<mailto:plantgenememberforu...@cornell.edu>
Subject: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

[External Email]
Hello, PlantGENE forum,

I was wondering if anyone can point me toward sources for Rhizobium rhizogenes 
(Agrobacterium rhizogenes) for plant transformation?

My location is at Cornell University in New York, USA, so an American source 
may be easiest.

Thank you,

Lena Wilson
lc...@cornell.edu<mailto:lc...@cornell.edu>
PhD student
Weber Lab
Cornell University

P.S. This seems to be the first PlantGENE forum message, so fingers crossed 
that this goes well!




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RE: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

2024-03-27 Thread Orbovic,Vladimir
Hello Lena,

The easiest way to get R rhizogenes would be from ATCC.org (in the state of 
Virginia). However, I hope you have a permit to use it. The APHIS has recently 
changed regulations in respect to this species and you have to receive permit 
to be able to use it. I am in the process of getting the permit now. Hope this 
helps.

Vladimir

Vladimir Orbovic PhD
Citrus Research and Education Center
University of Florida/IFAS
700 Experiment Station Road
Lake Alfred, FL 33850
Phone 863-956-8729
Fax 863-956-4631
For the podcast on The Use of Genetic Engineering in Fruit Breeding please 
click 
here<https://www.scipod.global/harnessing-genetics-for-sustainable-fruit-production-dr-vladimir-orbovic-university-of-florida/>
Google Scholar Profile click 
here<https://scholar.google.com/citations?user=3EkgV00J=en>


From: bounce-128114308-97181...@list.cornell.edu 
 On Behalf Of Lena Wilson
Sent: Wednesday, March 27, 2024 3:17 PM
To: plantgenememberforu...@cornell.edu
Subject: Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

[External Email]
Hello, PlantGENE forum,

I was wondering if anyone can point me toward sources for Rhizobium rhizogenes 
(Agrobacterium rhizogenes) for plant transformation?

My location is at Cornell University in New York, USA, so an American source 
may be easiest.

Thank you,

Lena Wilson
lc...@cornell.edu<mailto:lc...@cornell.edu>
PhD student
Weber Lab
Cornell University

P.S. This seems to be the first PlantGENE forum message, so fingers crossed 
that this goes well!



Source for Rhizobium rhizogenes (Agrobacterium rhizogenes)?

2024-03-27 Thread Lena Wilson
Hello, PlantGENE forum,

I was wondering if anyone can point me toward sources for Rhizobium
rhizogenes (Agrobacterium rhizogenes) for plant transformation?

My location is at Cornell University in New York, USA, so an American
source may be easiest.

Thank you,

Lena Wilson
lc...@cornell.edu
PhD student
Weber Lab
Cornell University

P.S. This seems to be the first PlantGENE forum message, so fingers crossed
that this goes well!