[ccp4bb] EM maps

Hi, I'm almost certain this has been addressed before so apologies... but is there a trick to import EM map files into COOT, it keeps crashing when I've tried. Ta muchly in advance Dean To unsubscribe from the CCP4BB

Re: [ccp4bb] FW: [ccp4bb] old data - headers

huge thanks everyone.. what a response. All good now :) -Original Message- From: Pedro Matias [mailto:mat...@itqb.unl.pt] Sent: den 31 januari 2019 11:46 To: Dean Derbyshire ; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] FW: [ccp4bb] old data - headers Hi Dean, I reckon

[ccp4bb] FW: [ccp4bb] old data - headers

- From: Dean Derbyshire Sent: den 31 januari 2019 10:50 To: 'graeme.win...@diamond.ac.uk' ; 'Luca Jovine' Subject: RE: [ccp4bb] old data ok may have lied.. not just 1 dataset i note. Daresbury 14-1 on 19th April 2005. (I'm assuming MAR image plate but!) ESRF ID23-1 on 4th September 2007. ESRF

[ccp4bb] old data

maybe a silly question it there a data base or other way to tell what detector was used to collect historic data. Image header isn't hugely helpful ESRF is all I know for the source but I'd like to know what the detector was at the time... - I'm talking 2005-2010 Dean Derbyshire

[ccp4bb] comparisons - views

the prevailing thoughts on SONICC vs standard UV laser technology... any experiences with coping phase separation or condensation ? Thanks Dean Dean Derbyshire [cid:image001.jpg@01CFE9DF.CC204740] Box 1086 SE-141 22 Huddinge SWEDEN Visit: Lunastigen 7 Direct: +46 8 54683219

[ccp4bb] metal ion dominating density!

Hi all, I recall there was a post a while back, but silly me can't remember the details - How does one stop metal ions dominating real space refinement in COOT? D. Dean Derbyshire Senior Research Scientist [cid:image001.jpg@01CF80A0.6F20B190] Box 1086 SE-141 22 Huddinge SWEDEN

Re: [ccp4bb] metals disapear

. From: Helland Ronny [ronny.hell...@uit.no] Sent: 01 May 2014 23:57 To: Dean Derbyshire Subject: RE: metals disapear Hi Dean, What is the metal and what is the pH where you crystallized your protein? We had a Mg binding protein (DNA binding protein) which crystallized both

[ccp4bb] metals disapear

Hi all, Has anyone experienced catalytic metal ions disappearing during data collection ? If so, is there a way of preventing it? D. Dean Derbyshire Senior Research Scientist [cid:image001.jpg@01CF6470.5FA976D0] Box 1086 SE-141 22 Huddinge SWEDEN Visit: Lunastigen 7 Direct

Re: [ccp4bb] sorry, sort of off topic

Thanks everyone for the info coming in.. another related: is there such a thing as a trade fair for crystallography... or should I attend the biggest conferences? ta again Dean From: Dean Derbyshire Sent: 27 March 2014 13:35 To: CCP4BB@JISCMAIL.AC.UK Subject

[ccp4bb] sorry, sort of off topic

I'm after suggestions as to where the best place(s) for obtaining info on latest equipment - advances in techniques etc. Oh, for protein crystallisation and screening ahead of data collection! cheers Dean

[ccp4bb] cyclising proteins

Hi all, perhaps not the right forum but... ...is there a way in coot to make a lysine side-chain // carboxy-terminal iso-peptide bond? Cheers in advance Dean

[ccp4bb] Is there a way of assessing how much of a structure is 'modelable'?

Hi all, I'm having great trouble building/refining a structure which has several regions of obvious flexibility/disorder... Regions where the density is fragmented and I have no clear way of building the chain (at least with any confidence). The protein is dimeric with some variation in the

[ccp4bb] rotomer library

Hi, potentially a very silly question but... I'm trying to find the 'rotomer library' file that for example COOT uses! Be very grateful to be pointed in the right direction. :0) Dean

Re: [ccp4bb] mosflm and pilatus 2M

Hi again, it's the 2M detector I'm having problems with. Got different data from a 6M and yep that works a dream. ? :0) From: Harry [ha...@mrc-lmb.cam.ac.uk] Sent: 12 March 2012 20:30 To: Dean Derbyshire Cc: Harry; CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb

[ccp4bb] Real space refinement in coot

Anyone know how to minimize the domination of heavy atoms during RSR in coot. The density from my zincs causes the coordinating His residues to collapse onto the metal ion when I try RSR. Cheers in advance Dean

Re: [ccp4bb] odd arginines

Hi all, Has anyone observed 'odd' arginine residues, missing the NH1 atom; and possibly related.. very close Chlorine-arginine (NH1 again) distances. I have a 1.3Å structure and am having trouble getting my head around some very odd density features. I have 2 molecules in the assym. with the

[COOT] Ligands

Perhaps (most likely in fact) this has been asked and addressed in the past: is there (or will there be) a way in COOT to 'build' ligands from scratch? Currently I go through a long winded route of Sketcher... importing and pulling the coords around to fit electron density... then finally