https://www.dropbox.com/scl/fo/pmw9nisdmq53yir2jqcmu/h?rlkey=zaj6cnknkjgkowrzf0vrmqdyf=0
Here is a link to my Molecular replacement and asymmetric unit contents
logfiles.
I ran Simple molecular replacement phaser MR through ccp4 cloud with my .mtz
that was auto-processed at the ALS light
Dear all,
The ESRF Algorithms & Scientific Data Analysis group is opening an exciting
position for a computational scientist in close collaboration with the
Structural Biology group.
Structural Biology is one of the most important family of techniques at the
ESRF, both for academic and
Dear Colleagues,
I am excited to let you know that the Department of Biochemistry, Microbiology
and Immunology, College of Medicine, University of Saskatchewan, is searching
for an exceptional candidate in the area of Protein Science for Pathogen
Defense, to fill a tenure-track Canada Research
-- Forwarded message -
From: Helen McAllister
Date: Fri, 16 Feb 2024 at 13:10
Subject: [CCP4] Please could you advertise another post for us?
To: c...@listserv.stfc.ac.uk
Dear Sirs,
Please could you advertise the following post on your website? (This is by
coincidence the
I got i2run to work with this data by:
* Importing the data with "Import merged"
* Importing the 2 sequences one at a time with 2 import sequence jobs
* Running a 'Define AU contents' job with both sequences added to the
contents.
* Creating a Crank2 job, then clicking the "Show i2 run command"
Hi Marco,
To follow up: if your protein is something like WRN or BLM helicase - you
mention recA like fold after all - (I worked on these in the past hehe)
then the split into domains is almost mandatory - these proteins are very
plastic and doing MR on them is equivalent to doing antibody MR
Hi Marco
Further to Pedro’s comment, one way to avoid missing data in cusps is to use a
multi-sweep strategy for data collection (as championed by the good folk at
Global Phasing over many years). Many of the problems encountered in structure
solution can be traced back to a sub-optimal data
Hmm - I can't help with the scripting question, but it solved easily from
the I2 interface?
This is run on a Mac.
CRANK2 called SHELX - found SE with weak signal..
Some difference between hand
It traced ALA - R factor 41%
Then BUCCANEER or MODELFIR quickly built and sequenced the rest..
Eleanor
Hi Marco,
I was just about to write with a different suggestion when Kay’s reply came in.
What Kay suggests sounds like the best thing to do first!
However, if that isn’t the issue, then I’m wondering if it could be a problem
with incorrectly diagnosed translational non-crystallographic
Hi Marco,
Further to Zhen's comment, that can also happen if you have a cusp
containing one of the reciprocal lattice axes. The SG may be P212121 but
one of the 21 is never located due to the missing data.
You will always sort of get a solution from MR but you should look at
the Z-score of
Hello Marco,
this sounds to me like there is space group confusion in the sense that if you
use the output model from Phaser, it may say space group P22121 (with a wrote:
>Hello Todd, I get the best solution for p22121 space group after MR with an
>LLG score of 640 from phaser. and the Rfree
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