Hi Chen,
I had a similar condition before (reservoir solution with 10% dioxane) and an
oil-based cryoprotectant (66.5% w/w paraton-N, 28.5% paraffin oil and 5%
glycerol) worked for me. Please check this paper out:
http://scripts.iucr.org/cgi-bin/paper?wd5052
In addition, have you tried
Chen,
Dioxane is not easy to work with, exactly for the reasons you describe.
There is one thing you did not mention, which I know to be an additional issue:
the quality of the dioxane. I do not know if you need good quality (whatever
that is) but it is a fact that crystallization works with
Dear Mark,
Thank you so much for your reply. Interestingly, after several days I
posted this question, I found both my previous crystals and precipitant
completely dissolved, but instead much larger crystals start to appear.
However, most of them are intergrown together, only few are single. And
Another thing to try when cryoprotecting any crystals grown at room temp using
a volatile precipitant is to first transfer the crystal tray to 4 degrees, then
quickly add a cold aliquot of a viscous cryoprotectant to the drop. Evaporation
is considerably slower at 4 degrees, and I prefer to
Dear all,
I am now optimizing a hit which contains about 30% 1,4-dioxane using
hanging-drop vapor diffusion at 25 degree. I am having a hard time to
reproduce the results: most of the times the drops are either dry in one
day or full of precipitate, and only occasionally could I get small
