Dear Morgan Elizabeth,
To complete the answers and suggestion made by the ccp4 community, I
would add, that you can actually combine the service offered by HTX
facility : You send your protein, and they are preparing the
crystallization plate for you in CD-plate which may be use in both
--
*From: *"Blake, Morgan Elizabeth"
*To: *"CCP4BB"
*Sent: *Wednesday, 22 November, 2023 17:44:29
*Subject: *[ccp4bb] Fragile Crystals
Hello!
I am a PhD student working on a crystallography project to wrap up my
dissertation research. I have purified a complex of two protei
: "Blake, Morgan Elizabeth"
To: "CCP4BB"
Sent: Wednesday, 22 November, 2023 17:44:29
Subject: [ccp4bb] Fragile Crystals
Hello!
I am a PhD student working on a crystallography project to wrap up my
dissertation research. I have purified a complex of two proteins, and I ca
Hi
Well worth contacting your favourite (or favorite…) synchrotron about in-plate
data collection - I’d imagine that APS has something in-place (there was a
report way back in the depths of time [2015, when I still worked in the field…]
about a prototype on ID-19?), and that might be easier to
Dear Elizabeth,
/In situ/ data collection is a good approach to try in your case. You
could use the CrystalDirect technology for automated crystal harvesting
that is more gentle to crystals than manual harvesting. This is
available both at EMBL Grenoble and EMBL Hamburg facilities, which
CCP4BB
<193323b1e616-dmarc-requ...@jiscmail.ac.uk>
Sent: Thursday, November 23, 2023 7:04:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fragile Crystals
Hi
Sorry if someone else has mentioned this already, but how about shooting your
crystals in situ (i.e. while sitting in t
Hi
Sorry if someone else has mentioned this already, but how about shooting your
crystals in situ (i.e. while sitting in the plate)? I know that VMXi at Diamond
can do this (in fact it's designed for his purpose) but haven't collected data
in long enough not to know if it can be done
Hello Morgan
In addition to the other good suggestions, I have a few observations of
my own.
If your crystals crack without handling or adding anything to the drop,
then they are extremely environment-sensitive. If that's the case,
testing at room temperature will be problematic because
11:44 AM
> To: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK>
> Subject: [ccp4bb] Fragile Crystals
>
> Hello!
>
> I am a PhD student working on a crystallography project to wrap up my
> dissertation research. I have purified a complex of two proteins, and I can
> cons
bulletin board On Behalf Of Blake, Morgan
Elizabeth
Sent: Wednesday, November 22, 2023 11:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fragile Crystals
Hello!
I am a PhD student working on a crystallography project to wrap up my
dissertation research. I have purified a complex of two
Hi Blake,
You can cross seed and get new condition with good stable crystal
system. Also you could try VMXi beamline, you do not need to use cryo and
use the plate directly.. You have to use Greiner Crystal QuickX ™ or
MiTeGen In-Situ-1 ™ plates.
Thanks Kannan
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On Wed, 22
ward Medical Research Institute
700 Ellicott Street | Buffalo, NY 14203
From: CCP4 bulletin board on behalf of "Blake, Morgan
Elizabeth"
Reply-To: "Blake, Morgan Elizabeth"
Date: Wednesday, November 22, 2023 at 11:54 AM
To: "CCP4BB@JISCMAIL.AC.UK"
Subject: [c
dern world
MacCHESS, Cornell University
schul...@cornell.edu
From: CCP4 bulletin board on behalf of Blake, Morgan
Elizabeth
Sent: Wednesday, November 22, 2023 11:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4b
Hi Morgan,
Have you tried MMS?
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157405/
If you can reproducibly grow crystals MMS might give you hits in different
conditions during screening. I’ve used this successfully multiple times for
crystals that wouldn’t optimize with standard methods. We
Hello!
I am a PhD student working on a crystallography project to wrap up my
dissertation research. I have purified a complex of two proteins, and I can
consistently grow crystals in 10% PEG3350, 0.2M KSCN, 0.1M BIS-TRIS propane pH
7.5. These crystals have sharp edges and can grow to a large
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