Hanging or sitting drops, it's there any other reason you're looking for
different cover slides?
There are hydrophilic/phobic finishings to pick from, plus oil is also an
alternative both to treat the surface or to screen in oil (micro)batch.
Plus if hanging is your thing, dispense on a
We used to use 1% solution of dimethyl dichloro silane in toluene to siliconize
glass coverslips. Soak them for 30 min, rinse well with deioinized water, and
oven/air dry.Uma.
--Uma Gabale, PhDResearch AssociateMolecular and Cellular Biochemistry
Indiana University Bloomington
On
Agree. Nowadays I hardly do hanging drops and crystal clear tapes are good to seal the sitting drop plates too. I mainly use the plastic cover slips to set the cryo drops on for crystal harvesting. They can be put on any plates and the drops are good to be viewed under the microscope.
Or do sitting drop, it's much easier all round. Frank
Sent from tiny silly touch screen
From: Minmin Yu
Sent: Thursday, 31 January 2019 21:42
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is there any alternative to siliconized glass coverslips
for
Yeah I used to use Fisher Scientific square plastic cover slips. Now I am using
Sigma 22x22mm (Z36,590-4, 100 each Lot10B084279) smaller sized ones. These
plastic ones are much more durable than the glass ones and are very easy to be
handled. They work very well for both hanging drops or for
Fisher Scientific makes 22 inch square plastic cover slips, which are
labeled "Fisherbrand Unbreakable
Cover Slips".
I and my colleagues have used them for over 15 years instead of siliconized
glass coverslips.
Just dip each one in ethanol to clean before use, dry with an air blower,
and use it
in my experience using silane with PAGE plates, rain-x is much better (at least
in that case).
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Goldman,
Adrian
Gesendet: Donnerstag, 31. Januar 2019 17:43
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Is there any
For the last 150,000 years we've been using the Hampton THICK siliconized
coverslips - I'm told they're one of the many crystallization tricks/gizmos
that Alan D'Arcy invented. We quite often recycle them - and I've never
seen any problems. The thick ones are very easy to use because you can jam
Yes, that was it! I just couldn’t remember the name.
> On 31 Jan 2019, at 17:04, Mathews, Irimpan I.
> <2add487f8799-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Long back we used Prosil solution (I think, 1% solution) for siliconization.
> Mathews
>
>
> -Original Message-
> From:
Long back we used Prosil solution (I think, 1% solution) for siliconization.
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman,
Adrian
Sent: Thursday, January 31, 2019 8:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is
When I was a graduate student, about 150,000 years ago, we took regular
coverslips and doused them in ?silane to make siliconised ones. You then let
them sit in a rack to dry. It was a bit tedious but not horrendously so.
After a while, I stopped doing it altogether, because IMHO it didn’t
plastic.
Plastic cover slips are no good for UV or polarization, but they are way better
than glass if you happen to want to try in-situ diffraction.
(https://doi.org/10.1107/S002188981254)
If you can't afford commercial ones, then you can always cut up some inkjet
transparency film
We used to use dichlorodimethylsilane in toluene to siliconize both
coverslips and the special glass capillaries for crystal mounting.
I don't have the protocol we used anymore, but the one listed on
protocolpedia sounds familiar.
yes, rain-x is excellent (also for PAGE gel plates). a little bottle costs
almost nothing and will last you a lifetime.
best
jon
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von
Nagarajan V
Gesendet: Donnerstag, 31. Januar 2019 16:14
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re:
I know of Rain-X being used.
V. Nagarajan
On Thu, Jan 31, 2019 at 1:17 AM wrote:
> A long time ago, before siliconized coverslips became commercially
> available, we used to siliconize coverslips ourselves. It is not really
> that much work and unsiliconized cover slips should be very cheap. If
Hi,
I believe that one can put a 50-100uL drop of fresh SigmaCote (in a tube cap)
with the glass pieces (surface well exposed), sealed in a dedicated (because
the container will be coated too) container (air-tight lunch boxes). After a
while the SigmaCote vapor should react with the glass
Hi Rajnandani,
The cheaper DIY protocol that Herman referred to, is actually still in use by
us. Use a siliconizing solution (e.g. Serva 35130.02 silicone solution in
isopropanol). Put about 20-30 microliters on the coverslips, preferrably with a
repeating pipette and in a fumehood. Then
A long time ago, before siliconized coverslips became commercially available,
we used to siliconize coverslips ourselves. It is not really that much work and
unsiliconized cover slips should be very cheap. If you wish, I could try to
find back the protocol.
Best,
Herman
Von: CCP4 bulletin
Dear All
I am a PhD student who requires lots of coverslips (!!) for setting up
hanging drop crystallization. The company sells it for a huge amount. Also
there is a wide monetary difference between a normal siliconized coverslip
and a 22mm siliconized circle coverslips. We tried to search for an
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