On Thu, Aug 27, 2009 at 10:12 AM, Diya Vaka<[email protected]> wrote:
>
> Dear Henrik,
>
> Thank you for the help.Now its running fine.

Good.

>
> I want to get the top 100 over expressed genes and under expressed
> genes from this data.
>
> So can I use the affy and limma packages for that or is there any
> thing specifically in aroma.affymetrix.

Please start a new thread with a descriptive subject line (and as
detailed question as possible) whenever the question changes.  This
new question has nothing to do with "Could not locate a file for this
chip type").  Your previous message was titled "aroma.affymetrix"
(which I changed); go to
http://groups.google.com/group/aroma-affymetrix/topics - imagine if
all people would write "aroma.affymetrix" in the subject line.  So,
please spend some time on the subject line.

I consider this new question to be answered in thread
'"gene-1-0-st-array-analysis" - Discussion' started on Aug 27, 2007 [
http://groups.google.com/group/aroma-affymetrix/browse_thread/thread/9ee424979095c462
].

Cheers,

/Henrik

>
> Thanks in advance,
>
> Diya
>
> On Aug 27, 12:39 am, Henrik Bengtsson <[email protected]> wrote:
>> Hi.
>>
>>
>>
>> On Wed, Aug 26, 2009 at 12:10 PM, Diya Vaka<[email protected]> wrote:
>>
>> > Hi all,
>>
>> > I am trying to analyze MoGene-1_0-st-v1 data and I these are the
>> > errors:
>>
>> > In MoGene-1_0-st-v1 directory I have the MoGene-1_0-st-v1.cdf file and
>> > I ran the following commands
>>
>> > setwd("C:/Documents and Settings/annotationData/chipTypes/MoGene-1_0-
>> > st-v1/")
>> >> getwd()
>> > [1] "C:/Documents and Settings/annotationData/chipTypes/MoGene-1_0-st-
>> > v1"
>> >> chipType <- "MoGene-1_0-st-v1"
>> >> cdf <- AffymetrixCdfFile$fromChipType(chipType)
>>
>> Not the cause of the problem, but please use byChipType() not
>> fromChipType() - who told you to use fromChipType()?
>>
>> > Error in list(`AffymetrixCdfFile$fromChipType(chipType)` =
>> > <environment>,  :
>>
>> > [2009-08-26 13:25:55] Exception: Could not locate a file for this chip
>> > type: MoGene-1_0-st-v1
>> >  at throw(Exception(...))
>> >  at throw.default("Could not locate a file for this chip type: ",
>> > paste(c(chipT
>> >  at throw("Could not locate a file for this chip type: ", paste(c
>> > (chipType, tag
>> >  at byChipType.UnitNamesFile(static, ...)
>> >  at byChipType(static, ...)
>> >  at method(static, ...)
>> >  at AffymetrixCdfFile$fromChipType(chipType)
>>
>> It is correct that annotation files for the 'MoGene-1_0-st-v1' chip
>> type should be located in a directory
>>
>>    annotationData/chipTypes/MoGene-1_0-st-v1/
>>
>> but it is also important to understand that this is the path
>> aroma.affymetrix uses to look up those files, that is, your working
>> directory should not be that directory but, in your case, C:/Documents
>> and Settings/.
>>
>> Here is a troubleshooting script:
>>
>> library("aroma.affymetrix")
>> stopifnot(isDirectory("annotationData"))
>> stopifnot(isDirectory("annotationData/chipTypes"))
>> stopifnot(isDirectory("annotationData/chipTypes/MoGene-1_0-st-v1/"))
>> stopifnot(isFile("annotationData/chipTypes/MoGene-1_0-st-v1/MoGene-1_0-st-v1.cdf"))
>>
>> (the above won't work if you use Windows Shortcut links, but I don't you do).
>>
>> You can read more on Page 'Setting up annotation files':
>>
>>  http://groups.google.com/group/aroma-affymetrix/web/setup
>>
>> Hope this helps
>>
>> Henrik
>>
>>
>>
>> > Can anyone help me
>>
>> > Thanks
>>
>> > Diya
> >
>

--~--~---------~--~----~------------~-------~--~----~
When reporting problems on aroma.affymetrix, make sure 1) to run the latest 
version of the package, 2) to report the output of sessionInfo() and 
traceback(), and 3) to post a complete code example.


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