Hi Jiang.

A couple quick comments below.

On 20-Nov-09, at 10:07 AM, camelbbs wrote:

> Hi,
> After I got the firma scores, how can i analyze it. I see boxplot of
> firma scores in the paper. So how i can get the same result.

You can use the boxplot() command on your matrix of FIRMA scores?   
That's how the boxplot in the paper was made.


> I want to check the alternative splicing between our several samples.
> Now I have got the score of each one but How to compare them.

You've read the paper.  So, you'll know that extreme FIRMA scores  
(i.e. large positive/negative) represent putative differential  
splicing events.  So, in general, you are looking for large (in  
magnitude) values.  If you have replicates, maybe you want to look for  
significant changes in FIRMA scores between groups.

Hope that helps.

Cheers,
Mark



> Thanks very much.
> Jiang
>
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------------------------------
Mark Robinson, PhD (Melb)
Epigenetics Laboratory, Garvan
Bioinformatics Division, WEHI
e: m.robin...@garvan.org.au
e: mrobin...@wehi.edu.au
p: +61 (0)3 9345 2628
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