Dear Usergrop. while proccessing data from the 5000k Sty array (but not the Nsp set), I receive the folleowing error:
> acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2") > csC <- process(acc) Error in sort.list(pairsToBuild) : 'x' must be atomic for 'sort.list' Have you called 'sort' on a list? Calls: process ... groupBySnpNucleotides.AromaCellSequenceFile -> sort -> sort.list In addition: Warning messages: 1: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'idxs' not found 2: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'seqsPP' not found 3: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'positions' not found 4: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'cellsPP' not found 5: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'snpPosition' not found Execution halted Any ideas what could be the cause? Thank you Karl-Heinz Session log: R version 2.10.1 (2009-12-14) Copyright (C) 2009 The R Foundation for Statistical Computing ISBN 3-900051-07-0 R is free software and comes with ABSOLUTELY NO WARRANTY. You are welcome to redistribute it under certain conditions. Type 'license()' or 'licence()' for distribution details. Natural language support but running in an English locale R is a collaborative project with many contributors. Type 'contributors()' for more information and 'citation()' on how to cite R or R packages in publications. Type 'demo()' for some demos, 'help()' for on-line help, or 'help.start()' for an HTML browser interface to help. Type 'q()' to quit R. Attempting to load the environment 'package:R.utils' Loading required package: R.oo Loading required package: R.methodsS3 R.methodsS3 v1.2.0 (2010-03-13) successfully loaded. See ?R.methodsS3 for help. Loading required package: utils R.oo v1.7.2 (2010-04-13) successfully loaded. See ?R.oo for help. R.utils v1.4.0 (2010-03-24) successfully loaded. See ?R.utils for help. [Previously saved workspace restored] > library("aroma.affymetrix") Loading required package: R.filesets Loading required package: digest R.filesets v0.8.1 (2010-04-22) successfully loaded. See ?R.filesets for help. Loading required package: aroma.core Loading required package: R.cache R.cache v0.3.0 (2010-03-13) successfully loaded. See ?R.cache for help. Loading required package: R.rsp R.rsp v0.3.6 (2009-09-16) successfully loaded. See ?R.rsp for help. Type browseRsp() to open the RSP main menu in your browser. Loading required package: matrixStats matrixStats v0.2.1 (2010-04-05) successfully loaded. See ?matrixStats for help. Loading required package: aroma.light aroma.light v1.15.1 (2009-11-01) successfully loaded. See ?aroma.light for help. aroma.core v1.5.0 (2010-02-22) successfully loaded. See ?aroma.core for help. Loading required package: aroma.apd Loading required package: R.huge R.huge v0.2.0 (2009-10-16) successfully loaded. See ?R.huge for help. Loading required package: affxparser aroma.apd v0.1.7 (2009-10-16) successfully loaded. See ?aroma.apd for help. aroma.affymetrix v1.5.0 (2010-02-22) successfully loaded. See ?aroma.affymetrix for help. > log <- verbose <- Arguments$getVerbose(-8, timestamp=TRUE) > options(digits=4) # Don't display too many decimals. > > chiptype='Mapping250K_Sty' > project<-"haprefTrio" > > cdf <- AffymetrixCdfFile$byChipType(chiptype) > cs <- AffymetrixCelSet$byName(project,chipType=chiptype) > print(cs) AffymetrixCelSet: Name: haprefTrio Tags: Path: rawData/haprefTrio/Mapping250K_Sty Platform: Affymetrix Chip type: Mapping250K_Sty Number of arrays: 8 Names: NA10851_FinSty_vR2_578246_A1_2_SC1, NA10855_FinSty_vR2_555824_A4_2_SC1, ..., NA12234_FinSty_vR2_554144_A12_2_SC2 Time period: 2005-08-31 15:29:36 -- 2005-09-01 15:14:51 Total file size: 501.33MB RAM: 0.02MB > gi <- getGenomeInformation(cdf) > print(gi) DChipGenomeInformation: Name: Mapping500K genome info hg17 Tags: Full name: Mapping500K genome info hg17 Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping500K genome info hg17.txt File size: 22.91 MB (24021904 bytes) RAM: 0.00 MB Chip type: Mapping250K_Sty > si <- getSnpInformation(cdf) 20100531 15:51:53|Defining DChipSnpInformation from chip type... 20100531 15:51:53| Chip type: Mapping250K_Sty 20100531 15:51:53| Version: 20100531 15:51:53| Located pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty snp info.txt 20100531 15:51:53| Instantiating DChipSnpInformation... DChipSnpInformation: Name: Mapping250K_Sty snp info Tags: Full name: Mapping250K_Sty snp info Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty snp info.txt File size: 24.23 MB (25402597 bytes) RAM: 0.00 MB Chip type: Mapping250K_Sty Number of enzymes: 1 20100531 15:51:53| Instantiating DChipSnpInformation...done 20100531 15:51:53|Defining DChipSnpInformation from chip type...done > print(si) DChipSnpInformation: Name: Mapping250K_Sty snp info Tags: Full name: Mapping250K_Sty snp info Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty snp info.txt File size: 24.23 MB (25402597 bytes) RAM: 0.00 MB Chip type: Mapping250K_Sty Number of enzymes: 1 > acs <- AromaCellSequenceFile$byChipType(getChipType(cdf, fullname=FALSE)) > print(acs) AromaCellSequenceFile: Name: Mapping250K_Sty Tags: Full name: Mapping250K_Sty, Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty,.acs File size: 162.50 MB (170393859 bytes) RAM: 0.00 MB Number of data rows: 6553600 File format: v1 Dimensions: 6553600x26 Column classes: raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw Number of bytes per column: 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1 Footer: <createdOn>20100529 10:27:19 EDT</createdOn><platform>Affymetrix</platform><chipType>Mapping250K_Sty</chipType> Chip type: Mapping250K_Sty Platform: Affymetrix > csR <- AffymetrixCelSet$byName(project, cdf=cdf) > print(csR) AffymetrixCelSet: Name: haprefTrio Tags: Path: rawData/haprefTrio/Mapping250K_Sty Platform: Affymetrix Chip type: Mapping250K_Sty Number of arrays: 8 Names: NA10851_FinSty_vR2_578246_A1_2_SC1, NA10855_FinSty_vR2_555824_A4_2_SC1, ..., NA12234_FinSty_vR2_554144_A12_2_SC2 Time period: 2005-08-31 15:29:36 -- 2005-09-01 15:14:51 Total file size: 501.33MB RAM: 0.02MB > acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2") > csC <- process(acc) Error in sort.list(pairsToBuild) : 'x' must be atomic for 'sort.list' Have you called 'sort' on a list? Calls: process ... groupBySnpNucleotides.AromaCellSequenceFile -> sort -> sort.list In addition: Warning messages: 1: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'idxs' not found 2: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'seqsPP' not found 3: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'positions' not found 4: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'cellsPP' not found 5: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) : object 'snpPosition' not found Execution halted > > sessionInfo() R version 2.10.1 (2009-12-14) x86_64-unknown-linux-gnu locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices datasets utils methods base other attached packages: [1] aroma.affymetrix_1.5.0 aroma.apd_0.1.7 affxparser_1.18.0 [4] R.huge_0.2.0 aroma.core_1.5.0 aroma.light_1.15.1 [7] matrixStats_0.2.1 R.rsp_0.3.6 R.cache_0.3.0 [10] R.filesets_0.8.1 digest_0.4.2 R.utils_1.4.0 [13] R.oo_1.7.2 R.methodsS3_1.2.0 > traceback() No traceback available > rawData/haprefTrio/Mapping250K_Sty: md5.lst NA10863_FinSty_vR2_554144_A10_2_SC1.EXP NA12056_FinSty_vR2_578246_A2_2_SC2.EXP NA10851_FinSty_vR2_578246_A1_2_SC1.CEL NA11831_FinSty_vR2_555824_A5_2_SC2.CEL NA12057_FinSty_vR2_578246_A3_2_SC3.CEL NA10851_FinSty_vR2_578246_A1_2_SC1.CHP NA11831_FinSty_vR2_555824_A5_2_SC2.CHP NA12057_FinSty_vR2_578246_A3_2_SC3.CHP NA10851_FinSty_vR2_578246_A1_2_SC1.EXP NA11831_FinSty_vR2_555824_A5_2_SC2.EXP NA12057_FinSty_vR2_578246_A3_2_SC3.EXP NA10855_FinSty_vR2_555824_A4_2_SC1.CEL NA11832_FinSty_vR2_555824_A6_2_SC3.CEL NA12234_FinSty_vR2_554144_A12_2_SC2.CEL NA10855_FinSty_vR2_555824_A4_2_SC1.CHP NA11832_FinSty_vR2_555824_A6_2_SC3.CHP NA12234_FinSty_vR2_554144_A12_2_SC2.CHP NA10855_FinSty_vR2_555824_A4_2_SC1.EXP NA11832_FinSty_vR2_555824_A6_2_SC3.EXP NA12234_FinSty_vR2_554144_A12_2_SC2.EXP NA10863_FinSty_vR2_554144_A10_2_SC1.CEL NA12056_FinSty_vR2_578246_A2_2_SC2.CEL NA10863_FinSty_vR2_554144_A10_2_SC1.CHP NA12056_FinSty_vR2_578246_A2_2_SC2.CHP annotationData/chipTypes/Mapping250K_Sty: 500K Sty Concordance.xls Mapping250K_Sty genome info hg17.txt Mapping250K_Sty.psi Mapping500K genome info hg17.txt AFFX_README-NetAffx-CSV-Files.txt Mapping250k_Sty_genome_info_hg18.txt Mapping250K_Sty.SMD na30 Mapping250K_Sty,.acs Mapping250K_Sty.GRC Mapping250K_Sty snp info.txt README.probeSeqFormat.txt Mapping250K_Sty.cdf Mapping250K_Sty.mdl Mapping250K_Sty.specialSNPs Sty_consensus.txt Mapping250K_Sty.chrx Mapping250K_Sty.na28.annot.csv Mapping250K_Sty.txt Mapping250K_Sty.cif Mapping250K_Sty.probe_tab Mapping250K_Sty.v1.5.birdseed.models annotationData/chipTypes/Mapping250K_Sty/na30: Mapping250K_Sty.na30.annot.csv -- When reporting problems on aroma.affymetrix, make sure 1) to run the latest version of the package, 2) to report the output of sessionInfo() and traceback(), and 3) to post a complete code example. 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