Dear Usergrop.

while proccessing data from the 5000k Sty array (but not the Nsp set),
I receive the folleowing error:

> acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2")
> csC <- process(acc)
Error in sort.list(pairsToBuild) :
  'x' must be atomic for 'sort.list'
Have you called 'sort' on a list?
Calls: process ... groupBySnpNucleotides.AromaCellSequenceFile -> sort
-> sort.list
In addition: Warning messages:
1: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'idxs' not found
2: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'seqsPP' not found
3: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'positions' not found
4: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'cellsPP' not found
5: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'snpPosition' not found
Execution halted

Any ideas what could be the cause?

Thank you
Karl-Heinz


Session log:
R version 2.10.1 (2009-12-14)
Copyright (C) 2009 The R Foundation for Statistical Computing
ISBN 3-900051-07-0

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

  Natural language support but running in an English locale

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

Attempting to load the environment 'package:R.utils'
Loading required package: R.oo
Loading required package: R.methodsS3
R.methodsS3 v1.2.0 (2010-03-13) successfully loaded. See ?R.methodsS3 for help.
Loading required package: utils
R.oo v1.7.2 (2010-04-13) successfully loaded. See ?R.oo for help.
R.utils v1.4.0 (2010-03-24) successfully loaded. See ?R.utils for help.
[Previously saved workspace restored]

> library("aroma.affymetrix")
Loading required package: R.filesets
Loading required package: digest
R.filesets v0.8.1 (2010-04-22) successfully loaded. See ?R.filesets for help.
Loading required package: aroma.core
Loading required package: R.cache
R.cache v0.3.0 (2010-03-13) successfully loaded. See ?R.cache for help.
Loading required package: R.rsp
R.rsp v0.3.6 (2009-09-16) successfully loaded. See ?R.rsp for help.
 Type browseRsp() to open the RSP main menu in your browser.
Loading required package: matrixStats
matrixStats v0.2.1 (2010-04-05) successfully loaded. See ?matrixStats for help.
Loading required package: aroma.light
aroma.light v1.15.1 (2009-11-01) successfully loaded. See ?aroma.light for help.
aroma.core v1.5.0 (2010-02-22) successfully loaded. See ?aroma.core for help.
Loading required package: aroma.apd
Loading required package: R.huge
R.huge v0.2.0 (2009-10-16) successfully loaded. See ?R.huge for help.
Loading required package: affxparser
aroma.apd v0.1.7 (2009-10-16) successfully loaded. See ?aroma.apd for help.
aroma.affymetrix v1.5.0 (2010-02-22) successfully loaded. See
?aroma.affymetrix for help.
> log <- verbose <- Arguments$getVerbose(-8, timestamp=TRUE)
> options(digits=4) # Don't display too many decimals.
>
> chiptype='Mapping250K_Sty'
> project<-"haprefTrio"
>
> cdf <- AffymetrixCdfFile$byChipType(chiptype)
> cs <- AffymetrixCelSet$byName(project,chipType=chiptype)
> print(cs)
AffymetrixCelSet:
Name: haprefTrio
Tags:
Path: rawData/haprefTrio/Mapping250K_Sty
Platform: Affymetrix
Chip type: Mapping250K_Sty
Number of arrays: 8
Names: NA10851_FinSty_vR2_578246_A1_2_SC1,
NA10855_FinSty_vR2_555824_A4_2_SC1, ...,
NA12234_FinSty_vR2_554144_A12_2_SC2
Time period: 2005-08-31 15:29:36 -- 2005-09-01 15:14:51
Total file size: 501.33MB
RAM: 0.02MB
> gi <- getGenomeInformation(cdf)
> print(gi)
DChipGenomeInformation:
Name: Mapping500K genome info hg17
Tags:
Full name: Mapping500K genome info hg17
Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping500K genome
info hg17.txt
File size: 22.91 MB (24021904 bytes)
RAM: 0.00 MB
Chip type: Mapping250K_Sty
> si <- getSnpInformation(cdf)
20100531 15:51:53|Defining DChipSnpInformation from chip type...
20100531 15:51:53| Chip type: Mapping250K_Sty
20100531 15:51:53| Version:
20100531 15:51:53| Located pathname:
annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty snp info.txt
20100531 15:51:53| Instantiating DChipSnpInformation...
  DChipSnpInformation:
  Name: Mapping250K_Sty snp info
  Tags:
  Full name: Mapping250K_Sty snp info
  Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty
snp info.txt
  File size: 24.23 MB (25402597 bytes)
  RAM: 0.00 MB
  Chip type: Mapping250K_Sty
  Number of enzymes: 1
20100531 15:51:53| Instantiating DChipSnpInformation...done
20100531 15:51:53|Defining DChipSnpInformation from chip type...done
> print(si)
DChipSnpInformation:
Name: Mapping250K_Sty snp info
Tags:
Full name: Mapping250K_Sty snp info
Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty snp info.txt
File size: 24.23 MB (25402597 bytes)
RAM: 0.00 MB
Chip type: Mapping250K_Sty
Number of enzymes: 1
> acs <- AromaCellSequenceFile$byChipType(getChipType(cdf, fullname=FALSE))
> print(acs)
AromaCellSequenceFile:
Name: Mapping250K_Sty
Tags:
Full name: Mapping250K_Sty,
Pathname: annotationData/chipTypes/Mapping250K_Sty/Mapping250K_Sty,.acs
File size: 162.50 MB (170393859 bytes)
RAM: 0.00 MB
Number of data rows: 6553600
File format: v1
Dimensions: 6553600x26
Column classes: raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw,
raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw, raw,
raw
Number of bytes per column: 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
Footer: <createdOn>20100529 10:27:19
EDT</createdOn><platform>Affymetrix</platform><chipType>Mapping250K_Sty</chipType>
Chip type: Mapping250K_Sty
Platform: Affymetrix
> csR <- AffymetrixCelSet$byName(project, cdf=cdf)
> print(csR)
AffymetrixCelSet:
Name: haprefTrio
Tags:
Path: rawData/haprefTrio/Mapping250K_Sty
Platform: Affymetrix
Chip type: Mapping250K_Sty
Number of arrays: 8
Names: NA10851_FinSty_vR2_578246_A1_2_SC1,
NA10855_FinSty_vR2_555824_A4_2_SC1, ...,
NA12234_FinSty_vR2_554144_A12_2_SC2
Time period: 2005-08-31 15:29:36 -- 2005-09-01 15:14:51
Total file size: 501.33MB
RAM: 0.02MB
> acc <- AllelicCrosstalkCalibration(csR, model="CRMAv2")
> csC <- process(acc)
Error in sort.list(pairsToBuild) :
  'x' must be atomic for 'sort.list'
Have you called 'sort' on a list?
Calls: process ... groupBySnpNucleotides.AromaCellSequenceFile -> sort
-> sort.list
In addition: Warning messages:
1: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'idxs' not found
2: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'seqsPP' not found
3: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'positions' not found
4: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'cellsPP' not found
5: In rm(idxs, seqsPP, positions, cellsPP, snpPosition, cells, pos) :
  object 'snpPosition' not found
Execution halted


>
> sessionInfo()
R version 2.10.1 (2009-12-14)
x86_64-unknown-linux-gnu

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=C              LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages:
[1] stats     graphics  grDevices datasets  utils     methods   base

other attached packages:
 [1] aroma.affymetrix_1.5.0 aroma.apd_0.1.7        affxparser_1.18.0
 [4] R.huge_0.2.0           aroma.core_1.5.0       aroma.light_1.15.1
 [7] matrixStats_0.2.1      R.rsp_0.3.6            R.cache_0.3.0
[10] R.filesets_0.8.1       digest_0.4.2           R.utils_1.4.0
[13] R.oo_1.7.2             R.methodsS3_1.2.0
> traceback()
No traceback available
>


rawData/haprefTrio/Mapping250K_Sty:
md5.lst
NA10863_FinSty_vR2_554144_A10_2_SC1.EXP
NA12056_FinSty_vR2_578246_A2_2_SC2.EXP
NA10851_FinSty_vR2_578246_A1_2_SC1.CEL
NA11831_FinSty_vR2_555824_A5_2_SC2.CEL
NA12057_FinSty_vR2_578246_A3_2_SC3.CEL
NA10851_FinSty_vR2_578246_A1_2_SC1.CHP
NA11831_FinSty_vR2_555824_A5_2_SC2.CHP
NA12057_FinSty_vR2_578246_A3_2_SC3.CHP
NA10851_FinSty_vR2_578246_A1_2_SC1.EXP
NA11831_FinSty_vR2_555824_A5_2_SC2.EXP
NA12057_FinSty_vR2_578246_A3_2_SC3.EXP
NA10855_FinSty_vR2_555824_A4_2_SC1.CEL
NA11832_FinSty_vR2_555824_A6_2_SC3.CEL
NA12234_FinSty_vR2_554144_A12_2_SC2.CEL
NA10855_FinSty_vR2_555824_A4_2_SC1.CHP
NA11832_FinSty_vR2_555824_A6_2_SC3.CHP
NA12234_FinSty_vR2_554144_A12_2_SC2.CHP
NA10855_FinSty_vR2_555824_A4_2_SC1.EXP
NA11832_FinSty_vR2_555824_A6_2_SC3.EXP
NA12234_FinSty_vR2_554144_A12_2_SC2.EXP
NA10863_FinSty_vR2_554144_A10_2_SC1.CEL  NA12056_FinSty_vR2_578246_A2_2_SC2.CEL
NA10863_FinSty_vR2_554144_A10_2_SC1.CHP  NA12056_FinSty_vR2_578246_A2_2_SC2.CHP

annotationData/chipTypes/Mapping250K_Sty:
500K Sty Concordance.xls           Mapping250K_Sty genome info
hg17.txt  Mapping250K_Sty.psi                   Mapping500K genome
info hg17.txt
AFFX_README-NetAffx-CSV-Files.txt
Mapping250k_Sty_genome_info_hg18.txt  Mapping250K_Sty.SMD
     na30
Mapping250K_Sty,.acs               Mapping250K_Sty.GRC
  Mapping250K_Sty snp info.txt          README.probeSeqFormat.txt
Mapping250K_Sty.cdf                Mapping250K_Sty.mdl
  Mapping250K_Sty.specialSNPs           Sty_consensus.txt
Mapping250K_Sty.chrx               Mapping250K_Sty.na28.annot.csv
  Mapping250K_Sty.txt
Mapping250K_Sty.cif                Mapping250K_Sty.probe_tab
  Mapping250K_Sty.v1.5.birdseed.models

annotationData/chipTypes/Mapping250K_Sty/na30:
Mapping250K_Sty.na30.annot.csv

-- 
When reporting problems on aroma.affymetrix, make sure 1) to run the latest 
version of the package, 2) to report the output of sessionInfo() and 
traceback(), and 3) to post a complete code example.


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