This doesn't sound good. It makes Oncoscan unusable for research projects.

Michael.

> On 11 Nov 2015, at 23:40, Keith C <keithchi...@gmail.com> wrote:
> 
> From what I understand, the OncoScan assay has a different capture method but 
> in the end still involves hybridization to probes on a chip.
> The output is two CEL files, A and C which represent AT and GC.  Using their 
> oncoscan software, it combines the two CEL files into a .OSCHP file
> which you can process with DNA Nexus.  One advantage is that they have 
> precharacterized normal diploid cells so that a hapmap reference sample is
> no longer required.  However, the process is a pain and involves a lot of 
> manual interface clicking, file matching, and manual generation of images.
> I have not found a way to simply export the segmentation data.  I was told by 
> Affy that OncoScan does not use a CDF file, but rather a PGR.CLF file.  I'm 
> trying
> to get a hold of one to see what the difference is and if its convertable 
> into CDF format.
> 
> What I mainly want is a chromosome explorer type output of
> 1. images of all chromosomes for all samples.  (that's 24 plots by hand per 
> sample using Nexus )
> 2. segmentation file including non-cnv altered segments.  Currently Nexus 
> only outputs regions of significance.
> 
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