Hi BASE2 users! I am using BASE 2.11 and have some questions. I want to calculate Ratios from 2 bioassays organized as following: - 1st bioassay = control - 2nd = treatment Labellings are performed as following: Cy5= pool of all samples extracts. Same in all arrays (serves for normalizations between arrays) Cy3= experimental condition.
Here are my questions: - is it possible to perform Rquantile normalization (cf normalizeBetweenArrays, R Bioconductors)? - And how to calculate ratios for each reporter? we must calculate ratios of cy3 intensities between the two bioassays. (e.g. ch(2)[biaoassay1] / ch(2)[bioassay2]). Could someone help me? thanks! yannick lippi -- ---------------------------------------------------------- Equipe Interactions Plantes-Microorganismes UMR 5546 CNRS/Université P. Sabatier Pôle de Biotechnologies Végétales 24, chemin de Borde Rouge B.P. 17 Auzeville 31 326 CASTANET-TOLOSAN FRANCE ---------------------------------------------------------- ------------------------------------------------------------------------------ Stay on top of everything new and different, both inside and around Java (TM) technology - register by April 22, and save $200 on the JavaOne (SM) conference, June 2-5, 2009, San Francisco. 300 plus technical and hands-on sessions. Register today. Use priority code J9JMT32. http://p.sf.net/sfu/p _______________________________________________ The BASE general discussion mailing list basedb-users@lists.sourceforge.net unsubscribe: send a mail with subject "unsubscribe" to basedb-users-requ...@lists.sourceforge.net
