After this

    library(RNAseqData.HNRNPC.bam.chr14)
    library(TxDb.Hsapiens.UCSC.hg19.knownGene)
    fls <- RNAseqData.HNRNPC.bam.chr14_BAMFILES
    exByGn <- exonsBy(TxDb.Hsapiens.UCSC.hg19.knownGene, "gene")

Rsamtools is loaded but not attached; it's summarizeOverlaps,GRanges,character-method is available (maybe it shouldn't be, but that's a different question). Invoking

    summarizeOverlaps(exByGn, fls)

results in

> summarizeOverlaps(exByGn, fls)
Error in get(as.character(FUN), mode = "function", envir = envir) :
  object 'obeyQname<-' of mode 'function' was not found

with

> traceback()
14: get(as.character(FUN), mode = "function", envir = envir)
13: match.fun(FUN)
12: lapply(as.list(X), FUN = FUN, ...)
11: lapply(as.list(X), FUN = FUN, ...)
10: lapply(X, FUN = FUN, ...)
9: lapply(X, FUN = FUN, ...)
8: endoapply(object, "obeyQname<-", value = value)
7: endoapply(object, "obeyQname<-", value = value)
6: `obeyQname<-`(`*tmp*`, value = FALSE)
5: `obeyQname<-`(`*tmp*`, value = FALSE)
4: BamFileList(reads, yieldSize = yieldSize, obeyQname = FALSE,
       asMates = !singleEnd)
3: .local(features, reads, mode, ignore.strand, ...)
2: summarizeOverlaps(exByGn, fls)
1: summarizeOverlaps(exByGn, fls)

and the parent.frame() of the environment (where the search at line 14 starts) is IRanges, but should be Rsamtools (where endoapply was invoked).

Martin
--
Computational Biology / Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N.
PO Box 19024 Seattle, WA 98109

Location: Arnold Building M1 B861
Phone: (206) 667-2793

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