Hi Nico,

This looks like the usual flakiness of the Ensembl Mart service. I see these errors on the build report every day for various packages. The exact set of packages varies.

I can reproduce on my laptop (Ubuntu 21.04):

  > library(biomaRt)

> ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl")

> ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl")

> ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl")

  Ensembl site unresponsive, trying uswest mirror

  Error in curl::curl_fetch_memory(url, handle = handle) :

    SSL certificate problem: unable to get local issuer certificate


See my sessionInfo() below.

Note that I had to try 3 times before getting the error.

I can also reproduce this with the Docker container for RELEASE_3_14 but this time it took me 12 attempts:

> library(biomaRt)
> ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl") > ensembl <- useMart(biomart = "ensembl", dataset = "dmelanogaster_gene_ensembl")
Ensembl site unresponsive, trying asia mirror
Error in curl::curl_fetch_memory(url, handle = handle) :
  SSL certificate problem: unable to get local issuer certificate

See my sessionInfo() below.

I'm not sure there's something we can do on our Linux builders about this, or if it would be a good idea at all to try to do something (the problem is real and can affect anybody using Ubuntu). Maybe discuss this with Mike Smith by opening an issue here https://github.com/grimbough/biomaRt

Best,
H.


sessionInfo() for my laptop session:


R version 4.1.1 (2021-08-10)

Platform: x86_64-pc-linux-gnu (64-bit)

Running under: Ubuntu 21.04



Matrix products: default

BLAS:   /home/hpages/R/R-4.1.1/lib/libRblas.so

LAPACK: /home/hpages/R/R-4.1.1/lib/libRlapack.so



locale:

 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C

 [3] LC_TIME=en_GB              LC_COLLATE=en_US.UTF-8

 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8

 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C

 [9] LC_ADDRESS=C               LC_TELEPHONE=C

[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C



attached base packages:

[1] stats     graphics  grDevices utils     datasets  methods   base



other attached packages:

[1] biomaRt_2.50.0



loaded via a namespace (and not attached):

 [1] KEGGREST_1.34.0        progress_1.2.2         tidyselect_1.1.1

 [4] purrr_0.3.4            vctrs_0.3.8            generics_0.1.1

 [7] stats4_4.1.1           BiocFileCache_2.2.0    utf8_1.2.2

[10] blob_1.2.2             XML_3.99-0.8           rlang_0.4.12

[13] pillar_1.6.4           glue_1.4.2             DBI_1.1.1

[16] rappdirs_0.3.3         BiocGenerics_0.40.0    bit64_4.0.5

[19] dbplyr_2.1.1           GenomeInfoDbData_1.2.7 lifecycle_1.0.1

[22] stringr_1.4.0          zlibbioc_1.40.0        Biostrings_2.62.0

[25] memoise_2.0.0          Biobase_2.54.0         IRanges_2.28.0

[28] fastmap_1.1.0          GenomeInfoDb_1.30.0    curl_4.3.2

[31] AnnotationDbi_1.56.0   fansi_0.5.0            Rcpp_1.0.7

[34] filelock_1.0.2         cachem_1.0.6           S4Vectors_0.32.0

[37] XVector_0.34.0         bit_4.0.4              hms_1.1.1

[40] png_0.1-7              digest_0.6.28          stringi_1.7.5

[43] dplyr_1.0.7            tools_4.1.1            bitops_1.0-7

[46] magrittr_2.0.1         RCurl_1.98-1.5         RSQLite_2.2.8

[49] tibble_3.1.5           crayon_1.4.1           pkgconfig_2.0.3

[52] ellipsis_0.3.2         xml2_1.3.2             prettyunits_1.1.1

[55] assertthat_0.2.1       httr_1.4.2             rstudioapi_0.13

[58] R6_2.5.1               compiler_4.1.1



sessionInfo() on my Docker session:

R version 4.1.1 (2021-08-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.3 LTS

Matrix products: default
BLAS/LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.8.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=C
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base

other attached packages:
[1] biomaRt_2.50.0      BiocManager_1.30.16

loaded via a namespace (and not attached):
 [1] KEGGREST_1.34.0        progress_1.2.2         tidyselect_1.1.1
 [4] purrr_0.3.4            vctrs_0.3.8            generics_0.1.1
 [7] stats4_4.1.1           BiocFileCache_2.2.0    utf8_1.2.2
[10] blob_1.2.2             XML_3.99-0.8           rlang_0.4.12
[13] pillar_1.6.4           glue_1.4.2             DBI_1.1.1
[16] rappdirs_0.3.3         BiocGenerics_0.40.0    bit64_4.0.5
[19] dbplyr_2.1.1           GenomeInfoDbData_1.2.7 lifecycle_1.0.1
[22] stringr_1.4.0          zlibbioc_1.40.0        Biostrings_2.62.0
[25] memoise_2.0.0          Biobase_2.54.0         IRanges_2.28.0
[28] fastmap_1.1.0          GenomeInfoDb_1.30.0    curl_4.3.2
[31] AnnotationDbi_1.56.1   fansi_0.5.0            Rcpp_1.0.7
[34] filelock_1.0.2         cachem_1.0.6           S4Vectors_0.32.0
[37] XVector_0.34.0         bit_4.0.4              hms_1.1.1
[40] png_0.1-7              digest_0.6.28          stringi_1.7.5
[43] dplyr_1.0.7            tools_4.1.1            bitops_1.0-7
[46] magrittr_2.0.1         RCurl_1.98-1.5         RSQLite_2.2.8
[49] tibble_3.1.5           crayon_1.4.2           pkgconfig_2.0.3
[52] ellipsis_0.3.2         xml2_1.3.2             prettyunits_1.1.1
[55] assertthat_0.2.1       httr_1.4.2             rstudioapi_0.13
[58] R6_2.5.1               compiler_4.1.1


On 03/11/2021 15:15, Nicolas Delhomme wrote:
Hej!

The easyRNASeq package fails with the following error on nebbiolo2:

easyRNASeq RUnit Tests - 20 test functions, 1 error, 0 failures
ERROR in test_getAnnotation_BiomaRt: Error in h(simpleError(msg, call)) :
   error in evaluating the argument 'table' in selecting a method for function 
'%in%': SSL certificate problem: unable to get local issuer certificate

Not exactly sure what I can do about that.

Best,

Nico
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Hervé Pagès

Bioconductor Core Team
hpages.on.git...@gmail.com

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